FastQCFastQC Report
Wed 15 May 2024
000000000-GLMFC_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GLMFC_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3753407
Sequences flagged as poor quality0
Sequence length8
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGACAC3134658.351479069549345No Hit
TCTTCACA3132678.346203862251016No Hit
TCCGTCTA2961937.89131048138398No Hit
TTCACGCA2852927.600881012903743No Hit
TGGCTTCA2557866.8147685556082775No Hit
ACAGATTC2204345.872904270706587No Hit
AGCCATGC2128025.669569007570988No Hit
AGCACCTC2019785.381191008595657No Hit
TATCAGCA1973035.256637502940661No Hit
AATCCGTC1901825.066916537428528No Hit
ACACGACC1797934.79012800903286No Hit
ATCATTCC922212.456994405349593No Hit
TCTTCACC81920.218255041353096No Hit
AAGGACCC75200.20035130749209984No Hit
TCCGTCTC72010.1918523624003472TruSeq Adapter, Index 14 (100% over 8bp)
TTCACGCC61960.165076688992161No Hit
TGGCTTCC58260.15521897838417203No Hit
TATCAGCC40100.10683626902171814No Hit
TCTTCCCC38370.10222712325095573No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)