FastQCFastQC Report
Sat 6 Apr 2024
000000000-GKFT8_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GKFT8_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2458410
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC62387025.37697129445455Illumina Single End PCR Primer 1 (100% over 8bp)
GATAGCGT1525266.204253969028763No Hit
TCTACACT1485566.04276747979385Illumina Single End PCR Primer 1 (100% over 8bp)
CTAGTATG1226374.988468156247331No Hit
GTCTAGTG1203094.893772804373558No Hit
ACGCGTGA1152254.686972474078774No Hit
CGATCTAC1102224.483466956284754No Hit
CGTTACTA1028664.184249169178453No Hit
AAGCAGCA967753.936487404460607No Hit
TACGAGAC936783.810511672178359TruSeq Adapter, Index 14 (100% over 8bp)
AGAGTCAC912683.7124808311062845No Hit
GTCAGATA818143.3279233325604762No Hit
TCGACGAG812733.3059172391911846No Hit
GATCGTGT808003.2866771612546324No Hit
ACGTCTCG780443.17457218283362No Hit
TGCGTCAC714972.908261844037406No Hit
CTACTATA697762.838257247570584No Hit
TGCGTACG175930.7156251398261478No Hit
TAGTGTAG170890.6951240842658466No Hit
GACACTGA162200.6597760341033432No Hit
GCTCTAGT159310.6480204685142023No Hit
CTAGAGCT146260.5949373782241367No Hit
CGTCGCTA131150.5334748882407735No Hit
ATATACAC97220.3954588534866031No Hit
GGTAAGCT93340.3796762948409744No Hit
ACGACGTG78620.31980019606168214No Hit
TGTGGCAT61610.2506091335456657No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)