FastQCFastQC Report
Sat 6 Apr 2024
000000000-GKFT8_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GKFT8_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2458410
Sequences flagged as poor quality0
Sequence length8
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTACG1358815.527190338470801No Hit
CTCGACTT1138294.630187804312543No Hit
GTAACGAG1131644.603137800448257No Hit
CGAAGTAT1081884.400730553487824No Hit
TAGCAGCT1062224.320760166123633No Hit
TCTCTATG1059604.31010287136808ABI Solid3 Adapter B (100% over 8bp)
ATAGTACC854833.477166135835764No Hit
ATAGCGCT675102.7460838509443097No Hit
TCTAGACT661682.6914957228452536No Hit
ACGTGCGC643332.6168539828588395No Hit
TCCTCATG640132.60383743964595No Hit
CGAGCTAG601262.445727116306881No Hit
GCGTATAC581692.366122819220553No Hit
TGCTCGTA550712.2401064102407653No Hit
CAGTAGGT536882.183850537542558No Hit
CTCTAGAG524862.1349571470991413No Hit
ACGCTACT498862.029197733494413No Hit
CGAGAGTT423841.7240411485472316TruSeq Adapter, Index 13 (100% over 8bp)
GACATAGT384841.5654020281401393No Hit
ACTCACTG375731.5283455566809443No Hit
TGAGTACG329871.3418022217612198No Hit
CTGCGTAG306501.246740779609585No Hit
AGTCGCAG234740.954844798060535No Hit
ACTGTGTA212780.86551877026208No Hit
CGCGATAT191830.7803010889151931No Hit
CTATCGTG189360.770253944622744No Hit
ACGTACGT183010.744424241684666No Hit
TACTAGGT171800.698825663742012No Hit
GCGATACG161330.6562371614173388No Hit
TAACGTCC157120.6391122717528809No Hit
CTACGACC138300.5625587269820738No Hit
GAGACTTA125400.5100857871551124No Hit
GTTACAGC111980.45549765905605655No Hit
ACGTGCTC74960.3049125247619396No Hit
CAGTAGTT58840.23934168832700808No Hit
AAGCGTTC56400.22941657412717978No Hit
ATAACGAG55330.22506416749036978No Hit
GACATATT43890.17853002550428937No Hit
AATCTACG38890.1581916767341493No Hit
AAGGCGTA36050.14663949463270975No Hit
CTGATGAG34050.13850415512465375No Hit
ACGATACG34040.13846347842711346No Hit
AACATAGT29470.11987422765120546No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)