FastQCFastQC Report
Sat 6 Apr 2024
000000000-GKFT8_l01.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GKFT8_l01.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2458410
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT49851620.277984550990276No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG47454519.30292343425222No Hit
GATGAGTCAGCGTCGAACTTGGACATGTCGAGAATATCGTTGAGCAGTTC774823.1517118788159824No Hit
GATGAGTCAGCGTCGAACGCCTGTTGCGCTGTCTGCACGATCCGCTGGAG681402.771710170394686No Hit
CGGTTCGTGCGCTTCTGCGGAACACAATCCGCAACAACAGAACTGGTTAG186530.7587424392188448No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCTAC157830.6420003172782408No Hit
GAGTAACAGGATTAGATACCCTGAACTTTGTAGCCAATTTCTCAGCACGT156100.6349632486037724No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCTAC141600.5759820371703662No Hit
GAGTAACCGGATTAGATACCCTGAACTTTGTAGCCAATTTCTCAGCACGT137600.5597113581542541No Hit
GAGTAACAGGATTAGATACCCGGAACTTTGTAGCCAATTTCTCAGCACGT112800.45883314825435956No Hit
ACGCCCAAGCAGATTTACATCACGGTAGTTGAGATCGGACTTCATAAGGT111920.4552535988708149No Hit
GAGTAACCGGATTAGATACCCGGAACTTTGTAGCCAATTTCTCAGCACGT109390.444962394393124No Hit
ACGCCCAAGCAGATCTACATCACGGTAGTTGAGATCGGACTTCATAAGGT96770.39362840209729055No Hit
CGGTTCGTCCGCTTCTGCGGAACACAATCCGCAACAACAGAACTGGTTAG86830.3531957647422521No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAAC68860.2800997392623688No Hit
ACACCCAAGCAGATTTACATCACGGTAGTTGAGATCGGACTTCATAAGGT66910.2721677832420141No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATAAC61170.2488193588538934No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAAC60390.24564657644575152No Hit
ACACCCAAGCAGATCTACATCACGGTAGTTGAGATCGGACTTCATAAGGT59840.24340935808103611No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATAAC54750.22270491903303352No Hit
GATGAGTCAGCGTCGAACGCGGCCTGACGCGGCGGGCCGTCCAGGTTCAC50840.206800330294784No Hit
GATGAGTCAGCGTCGAACATGGACATGTCGAGAATATCGTTGAGCAGTTC50260.2044410818374478No Hit
GAGTAACAGGATTAGATACCCGGGTAGTCCACGCCCTAAACGATGTCTAC42660.1735267917068349No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACACCGTAAACGTTGGGCGC42180.17157431022490147No Hit
GATGAGTCAGCGTCGAGCGGTTCAGGTCGGTGTAGTGCGCCAGGCGCTCG37310.15176475852278506No Hit
GAGTAACCGGATTAGATACCCGGGTAGTCCACGCCCTAAACGATGTCTAC37260.15156137503508366No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGC36770.14956821685560992No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACACCGTAAACGTTGGGCGC36190.1472089683982737No Hit
GATGAGTCAGCGTCGAACACGTGCATTGCATCCGGCAGATCCTGCATTGG36100.14684287812041116No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGC34060.13854483182219401No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGC33450.13606355327223693No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGC29490.11995558104628602No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGATGC27260.11088467749480356No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGGCTCG26200.10657294755553387No Hit
CTGTAAAGATAAATGGGTCATCTAAGGAGTATGGAGCTGGGATTGACTCG26100.10616618058013107No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATTGC24750.10067482641219325No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGGGCTGGGATTGACTCG24690.10043076622695156No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)