FastQCFastQC Report
Thu 25 Jan 2024
000000000-GKBG2_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GKBG2_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4076423
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC93115922.842550932520986Illumina Single End PCR Primer 1 (100% over 8bp)
ATCGTACG3561528.736875442023559RNA PCR Primer, Index 22 (100% over 8bp)
TAGCGAGT2745306.734580783201351No Hit
CTGCGTGT2707366.641508989621538No Hit
TCATCGAG2586236.344361220609343No Hit
GACACCGT2475256.072112732167393No Hit
ACTATCTG2390705.864700498451706No Hit
CGTGAGTG2388595.859524391850404No Hit
GGATATCT2245425.5083096135999625No Hit
ACGCGTGA796981.9550964166378222No Hit
CGATCTAC794901.9499939039692396No Hit
AAGCAGCA720931.768535797192784No Hit
CTAGTATG644701.5815336141514265No Hit
GTCTAGTG587161.440380451194589No Hit
GATAGCGT562831.380695771758721No Hit
TCTACACT554741.3608499412352446Illumina Single End PCR Primer 1 (100% over 8bp)
TGCGTCAC516791.2677536163445255No Hit
AGAGTCAC363550.8918358080110921No Hit
TCGACGAG345620.847851167555477No Hit
GTCAGATA345330.8471397595391842No Hit
GATCGTGT324820.7968260408696547No Hit
TACGAGAC318310.7808561574694285TruSeq Adapter, Index 14 (100% over 8bp)
CTACTATA307860.755220937571984No Hit
ACGTCTCG298390.7319897861433909No Hit
CGTTACTA292380.717246468288497No Hit
ATCGCACG43070.10565635607492156No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)