FastQCFastQC Report
Thu 25 Jan 2024
000000000-GKBG2_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GKBG2_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4076423
Sequences flagged as poor quality0
Sequence length8
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTAGACT42164610.34352911854339No Hit
ATAGCGCT42037410.312325291070138No Hit
CAGTAGGT3832229.400938028266449No Hit
CATCGTGA2008414.9268930138015605No Hit
TCAGTCTA1710934.197135576950674No Hit
CGTCATAC1603913.934601487627756No Hit
ATGAGCTC1536743.7698246722678195No Hit
CTCTAGAG1514173.714457503551521No Hit
CGAGCTAG1509373.702682474316331No Hit
TCCTCATG912532.238555714164109No Hit
AACGCTGA854662.0965930179473524No Hit
CGTAGCGA766941.881404358674259No Hit
TGCTCGTA694621.7039939181974No Hit
AGCATACC327910.8044062159398081No Hit
GAGCTCGA313860.7699397241159713No Hit
TAGCAGCT285100.6993876739484592No Hit
CGAAGTAT254390.6240520181541513No Hit
CTACGACC191170.46896507060233933No Hit
ATAGTACC182020.44651892112275887No Hit
CTCGACTT175140.4296413792189868No Hit
ACGTGCGC170870.41916650946184925No Hit
TACTAGGT161030.39502769952971023No Hit
TCTCTATG158100.38784002543406315ABI Solid3 Adapter B (100% over 8bp)
GATCTACG152560.3742496791917816No Hit
CTCTCTCT139640.3425552255003958No Hit
CGCGATAT139020.3410342842241838No Hit
GAGACTTA138000.33853209051170596No Hit
ACTCACTG133030.32634002899110326No Hit
ACGTACGT131460.32248861317875993No Hit
GCGTATAC129590.31790125803921726No Hit
ACTACGAC127590.31299499585788815No Hit
GTAACGAG127180.3119892121107157No Hit
TAACGTCC121030.2969024559031288No Hit
CTATCGTG110970.2722239571310436No Hit
GCGATACG101250.2483795229297843No Hit
TCTCTCCT88790.21781350954010414No Hit
GACATAGT87890.2156056915585061No Hit
GTCTATGA87440.21450178256770705No Hit
CTGCGTAG87230.21398662503866747No Hit
AGTCGCAG83180.2040514441214761No Hit
ACGCTACT80640.1978204911511882No Hit
GTTACAGC80370.19715814575670876No Hit
TGCGTCAA76170.1868549951759177No Hit
CGAGAGTT75880.18614358715962498TruSeq Adapter, Index 13 (100% over 8bp)
CGAGCGAC67820.16637135056886884No Hit
ACTGTGTA65470.16060649250580716No Hit
CCTTCTTT64290.15771179781882302No Hit
TAGTCTCC63840.15660788882802398No Hit
CTAGCGCT63210.15506241624090533No Hit
TGAGTACG53690.13170860825777894No Hit
GTCTGCTA52390.12851953783991504No Hit
TATAGCGA51500.1263362511692236No Hit
TCTATACT41600.10205025337164471No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)