FastQCFastQC Report
Fri 1 Dec 2023
000000000-GK3C7_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GK3C7_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3799825
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC59747915.723855703881101Illumina Single End PCR Primer 1 (100% over 8bp)
GTCTAGTG1385933.647352180692532No Hit
CTAGTATG1341923.531531057351325No Hit
TCTACACT1314993.4606593724711003Illumina Single End PCR Primer 1 (100% over 8bp)
GATAGCGT1305693.4361845611311046No Hit
ACGCGTGA1164313.064114794760285No Hit
CGTTACTA1156243.0428769745975144No Hit
AAGCAGCA1134462.9855585454593303No Hit
GATCGTGT1060562.791075904811406No Hit
CTACTATA1052052.768680136585237No Hit
TCGACGAG1044582.749021336508918No Hit
ACGTCTCG988842.602330370477588No Hit
CGATCTAC974832.5654602514589486No Hit
GTCAGATA863252.2718151493818795No Hit
TACGAGAC845182.224260327778253TruSeq Adapter, Index 14 (100% over 8bp)
TGCGTCAC828682.1808372754008407No Hit
AGAGTCAC806672.122913555229517No Hit
ACTATCTG674151.7741606521352957No Hit
TGCGTACG659361.73523780700427No Hit
TAGCGAGT641031.686998743363181No Hit
CTAGAGCT623801.6416545498805866No Hit
GGATATCT621351.6352068845275771No Hit
GACACCGT605381.5931786332265303No Hit
GCTCTAGT595621.567493239820255No Hit
TCATCGAG594801.5653352457021048No Hit
ATATACAC594061.5633877875954814No Hit
GACACTGA591641.5570190732467943No Hit
TAGTGTAG566421.490647595612956No Hit
CGTCGCTA564501.4855947313363116No Hit
ACGACGTG542231.4269867691275255No Hit
ATCGTACG531601.399011796595896RNA PCR Primer, Index 22 (100% over 8bp)
CTGCGTGT518001.363220674636332No Hit
CGTGAGTG515061.3554834762127204No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)