FastQCFastQC Report
Fri 1 Dec 2023
000000000-GK3C7_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GK3C7_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3799825
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCATAC1016162.674228418414006No Hit
GACATAGT991092.6082516958017803No Hit
TAGTCTCC976742.5704867987341524No Hit
CTGCGTAG959742.525747896284697No Hit
GTCTGCTA913112.4030317185659866No Hit
GTCTATGA900622.3701617837663576No Hit
ACTCACTG887672.3360812669004494No Hit
CGAGAGTT861222.266472798089386TruSeq Adapter, Index 13 (100% over 8bp)
TGAGTACG787232.0717533044284933No Hit
TATAGCGA776612.0438046488983046No Hit
ACGCTACT716921.8867184672978359No Hit
AACGCTGA684991.8026882816971834No Hit
CGAGCGAC683631.7991091695012271No Hit
TCAGTCTA654391.7221582572881649No Hit
ACTACGAC646661.701815215174383No Hit
CGAAGTAT630401.6590237708315514No Hit
CGAGCTAG619141.6293908272091477No Hit
CGTAGCGA604811.5916785641444016No Hit
CTCGACTT594911.5656247327179542No Hit
ATAGTACC582921.534070647990368No Hit
CATCGTGA544481.4329080944517183No Hit
GTAACGAG529981.3947484423624772No Hit
TCTCTATG506491.332929805977907ABI Solid3 Adapter B (100% over 8bp)
TGCGTCAA500041.3159553400485549No Hit
TAGCAGCT484681.2755324258354004No Hit
CAGTAGGT483701.2729533596941964No Hit
ACGTGCGC465131.2240826880185272No Hit
GAGACTTA442481.1644746797549888No Hit
TGCTCGTA434491.143447395603745No Hit
CTCTAGAG412791.0863395024770877No Hit
GATCTACG405891.0681807714828973No Hit
TAACGTCC391181.0294684623633983No Hit
ATGAGCTC382841.0075200831617246No Hit
TCTAGACT362730.9545965932641634No Hit
GCGTATAC359060.9449382537353693No Hit
CTACGACC350580.9226214365135236No Hit
TACTAGGT349580.919989736369438No Hit
CGCGATAT328610.8648029843479633No Hit
GAGCTCGA318060.8370385478278606No Hit
GTTACAGC313370.8246958741520991No Hit
ATAGCGCT310710.8176955517688314No Hit
ACTGTGTA283380.7457711868309725No Hit
AGTCGCAG269230.7085326297921615No Hit
AGCATACC254690.6702677096971571No Hit
TCCTCATG250060.6580829380300408No Hit
GCGATACG239810.6311080115531637No Hit
ACGTACGT201550.5304191640404492No Hit
CGAGAATT148720.39138644542840784Illumina Small RNA Adapter 2 (100% over 8bp)
CTATCGTG138510.3645167869572941No Hit
TGAGTGCG128790.33893666155678226No Hit
CGAGCAAC119480.31443553321534545No Hit
ACGCTGCT115760.3046456086793471No Hit
ACTACAAC112590.2963031192225958No Hit
GACATGGT104110.27398630200075No Hit
ATAGTGCC95580.2515378997717No Hit
GTAACAAG91000.23948471311178804No Hit
CAGTAAGT85730.225615653352457No Hit
TAGCAACT83310.2192469390037699No Hit
GACATCGT76290.20077240399228913No Hit
TGCTCATA76230.20061450198364397No Hit
TCTCTGTG75180.19785121683235413No Hit
CTCTAAAG71280.18758758627042035No Hit
GATCTGCG66640.17537649760186325No Hit
TACTAAGT60810.1600336857618443No Hit
TCTAGGCT60220.1584809826768338No Hit
CTACGGCC56330.14824366911634088No Hit
ATAGCACT55140.14511194594487906No Hit
ACTGTATA48030.12640055792043056No Hit
AGCATGCC41330.10876816695505713No Hit
GCGATGCG38650.10171521056890778No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)