FastQCFastQC Report
Tue 17 Oct 2023
000000000-GHTTR_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GHTTR_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6191321
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCATAC2492734.026168244224455No Hit
GACATAGT2400933.8778961711079107No Hit
TGAGTACG1761082.8444333608288117No Hit
TCAGTCTA1726152.788015675491547No Hit
ACTCACTG1687572.725702640841914No Hit
CTGCGTAG1654062.6715784886617895No Hit
ACGCTACT1627732.6290512154029813No Hit
CGAGAGTT1567362.5315437529406086TruSeq Adapter, Index 13 (100% over 8bp)
TAGTCTCC1555192.5118872046853977No Hit
GTCTATGA1539892.4871751924993064No Hit
CGAGCTAG1533252.4764505022433827No Hit
GTCTGCTA1464232.364971869492795No Hit
CATCGTGA1351822.1834112623138098No Hit
ACTACGAC1325682.141190870252083No Hit
CGAGCGAC1313882.122131932749085No Hit
TATAGCGA1277622.0635660790322454No Hit
CAGTAGGT1186371.9161823462230436No Hit
CTCTAGAG1120841.810340636513597No Hit
ATAGTACC1059851.7118317722502194No Hit
AACGCTGA1053421.7014462664752805No Hit
GTAACGAG964831.5583588704252291No Hit
CGAAGTAT959741.5501376846718171No Hit
ATGAGCTC934891.5100008544218593No Hit
AGCATACC913311.4751456110901051No Hit
CGTAGCGA908621.4675704910147607No Hit
TCTCTATG901971.4568296491168848ABI Solid3 Adapter B (100% over 8bp)
TAGCAGCT875441.4139793430190424No Hit
TCTAGACT874051.4117342647877569No Hit
CTCGACTT873381.4106521047769933No Hit
GAGCTCGA850911.3743593653115385No Hit
TGCGTCAA818141.3214304346358394No Hit
TGCTCGTA790471.276738841355504No Hit
GAGACTTA777021.2550148829304764No Hit
ACGTGCGC738541.1928633647003604No Hit
ATAGCGCT731381.181298789062948No Hit
GATCTACG711111.1485594108268655No Hit
TAACGTCC674081.088749880679745No Hit
CTACGACC670981.0837428716747202No Hit
TACTAGGT659641.0654269097015No Hit
TCCTCATG654161.056575809911972No Hit
GTTACAGC617070.9966693699131413No Hit
CGCGATAT597280.9647052704907402No Hit
ACTGTGTA567570.9167187422522591No Hit
GCGTATAC551580.8908922667715017No Hit
GCGATACG513330.8291122363062746No Hit
AGTCGCAG451070.7285521135150318No Hit
ACGTACGT327970.5297254010896867No Hit
CTATCGTG274870.44396018232619505No Hit
TTTTTTTT103460.16710488763221937No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)