FastQCFastQC Report
Wed 2 Aug 2023
000000000-GG345_l01_n02_Plate2Col9_mod_third.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GG345_l01_n02_Plate2Col9_mod_third.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225171
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG17470077.58547948003961No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC75063.3334665654102884No Hit
GCTCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG67663.004827442254997No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGCAAGGTACACGCG22500.9992405771613575No Hit
GATCCTCCACATCTCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG19920.8846609909801884No Hit
GATCCTCCACATATCGCACCCTCAATGAATGGCCGGGAAAGGTACACGCG19250.8549058271269391No Hit
TATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG18850.8371415501996261No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAACGGTACACGCG13950.6195291578400416No Hit
GATCCTCCACATATCGAACCCTCACTGAATGGCCGGGAAAGGTACACGCG12300.5462515155148754No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGAACACGCG8680.3854848093226925No Hit
GATCCTCCACATATCGACCCCTCAATGAATGGCCGGGAAAGGTACACGCG7900.35084446931443214No Hit
GATCCTCCACATATCGAACCCTCAATGACTGGCCGGGAAAGGTACACGCG7860.3490680416217008No Hit
GATCCTCCACCTATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG6900.3064337769961496No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGACAGGTACACGCG6880.305545563149784No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACCCGCG4540.20162454312500278No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGACGGGAAAGGTACACGCG4280.1900777631222493No Hit
GATCCTCCCCATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG4220.18741312158315238No Hit
GATCCTCCACATATCGAACCCTCAATGAATGTCCGGGAAAGGTACACGCG3800.1687606308094737No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGGAAGGTACACGCG3010.13367618387803046No Hit
GATCCTCCACATATCGAACCCTCAATGAATTGCCGGGAAAGGTACACGCG2700.11990886925936288No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGAG2540.1128031584884377No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGCACACGCG2510.11147083771888919No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAAGTACACGCG2370.10525334079432963No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)