FastQCFastQC Report
Wed 2 Aug 2023
000000000-GG345_l01_n02_Plate2Col8_mod_third.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GG345_l01_n02_Plate2Col8_mod_third.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341768
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG14925143.6702675499169No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC12472136.492884061702675No Hit
GCTCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG62821.8380889960441003No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGCAAGGTACACGCG23330.6826268111701504No Hit
GATCCTCCACATATCGCACCCTCAATGAATGGCCGGGAAAGGTACACGCG20200.5910442171297489No Hit
GCTCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC19730.5772921982163339No Hit
GATCCTCCACATATCGAACCAGCTCTTGATTCAGGTAACCAAAAGCTAAC17930.5246248917394255No Hit
GATCCTCCACATCTCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG16230.47488354673345656No Hit
TATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG15580.4558647971723508No Hit
TATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC14590.4268977786100513No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAACGGTACACGCG13560.3967603754593759No Hit
GATCCTCCACATATCGAACCCTCACTGAATGGCCGGGAAAGGTACACGCG13070.38242316425177314No Hit
GATCCTCCACATATCGCACCAGATCTTGATTCAGGTAACCAAAAGCTAAC11770.3443856651295616No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGAACACGCG11310.33092624236324053No Hit
GATCCTCCACATATCGAACCCGATCTTGATTCAGGTAACCAAAAGCTAAC9550.279429320474708No Hit
GATCCTCCACATATCGAACCCTCAATGACTGGCCGGGAAAGGTACACGCG9540.27913672432761405No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAACGCTAAC9470.2770885512979565No Hit
GATCCTCCACATATCGACCCCTCAATGAATGGCCGGGAAAGGTACACGCG8760.2563142248542871No Hit
GATCCTCCACCTATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG7810.2285175908803633No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGACAGGTACACGCG6900.2018913414948152No Hit
GATCCTCCACATCTCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC6730.1969172069942183No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGGAACCAAAAGCTAAC6380.18667634184593057No Hit
GATCCTCCACATATCGAACCAGATCTTGCTTCAGGTAACCAAAAGCTAAC4630.13547201610449194No Hit
GATCCTCCCCATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG4590.1343016315161162No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACCCGCG4560.13342384307483438No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGACGGGAAAGGTACACGCG4430.12962009316261322No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAACAGCTAAC4030.11791624727885584No Hit
GATCCTCCACATATCGAACCCTCAATGAATGTCCGGGAAAGGTACACGCG3990.1167458626904801No Hit
GATCCTCCACATATCGACCCAGATCTTGATTCAGGTAACCAAAAGCTAAC3920.11469768966082254No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGCACACGCG3630.10621240139509842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)