FastQCFastQC Report
Wed 2 Aug 2023
000000000-GG345_l01_n02_Plate2Col7_mod_third.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GG345_l01_n02_Plate2Col7_mod_third.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274709
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG23397785.1726736291858No Hit
GCTCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG68082.478258812052026No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGCAAGGTACACGCG20100.7316833449213532No Hit
GATCCTCCACATATCGCACCCTCAATGAATGGCCGGGAAAGGTACACGCG17850.6497784928779181No Hit
TATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG16350.5951752581822947No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAACGGTACACGCG16010.5827985249846201No Hit
GATCCTCCACATATCGAACCCTCACTGAATGGCCGGGAAAGGTACACGCG13470.4903370475666979No Hit
GATCCTCCACATCTCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG12500.4550269557968614No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGAACACGCG12260.4462904382455617No Hit
GATCCTCCACATATCGACCCCTCAATGAATGGCCGGGAAAGGTACACGCG7990.2908532301453538No Hit
GATCCTCCACCTATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG7720.2810246479001416No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGACAGGTACACGCG7300.26573574218536705No Hit
GATCCTCCACATATCGAACCCTCAATGACTGGCCGGGAAAGGTACACGCG7250.26391563436217963No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGACGGGAAAGGTACACGCG5310.19329545082250674No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC5280.19220338612859425No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACCCGCG4640.16890600599179495No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGCACACGCG3550.12922765544630863No Hit
GATCCTCCCCATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG2800.10192603809849696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)