FastQCFastQC Report
Wed 2 Aug 2023
000000000-GG345_l01_n02_Plate2Col6_mod_third.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GG345_l01_n02_Plate2Col6_mod_third.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149832
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG13015786.86862619467136No Hit
GCTCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG43922.9312830370014415No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGCAAGGTACACGCG14970.9991190132948903No Hit
GATCCTCCACATATCGCACCCTCAATGAATGGCCGGGAAAGGTACACGCG10890.7268140317155214No Hit
GATCCTCCACATCTCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG9730.6493939879331518No Hit
TATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG8420.5619627315927171No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAACGGTACACGCG4700.31368466015270435No Hit
GATCCTCCACATATCGAACCCTCAATGACTGGCCGGGAAAGGTACACGCG4110.2743072240909819No Hit
GATCCTCCACATATCGAACCCTCACTGAATGGCCGGGAAAGGTACACGCG3960.2642960115329169No Hit
GATCCTCCACATATCGACCCCTCAATGAATGGCCGGGAAAGGTACACGCG3950.2636285973623792No Hit
GATCCTCCACCTATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG3470.23159271717657107No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGAACACGCG3450.23025788883549578No Hit
GATCCTCCACATATCGAACCCTCAATGAATGTCCGGGAAAGGTACACGCG2540.1695231993165679No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGACAGGTACACGCG2250.15016818837097548No Hit
GATCCTCCCCATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG1760.11746489401462971No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGGAAGGTACACGCG1730.11546265150301671No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACCCGCG1580.10545143894495168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)