FastQCFastQC Report
Wed 2 Aug 2023
000000000-GG345_l01_n02_Plate2Col5_mod_third.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GG345_l01_n02_Plate2Col5_mod_third.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300848
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG25038583.22641333829708No Hit
GCTCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG82102.7289528266765943No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGCAAGGTACACGCG25490.8472717119608573No Hit
GATCCTCCACATATCGCACCCTCAATGAATGGCCGGGAAAGGTACACGCG23130.7688267829601659No Hit
TATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG20670.6870579162899537No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAACGGTACACGCG19230.6391932138488539No Hit
GATCCTCCACATCTCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG17390.5780327607296708No Hit
GATCCTCCACATATCGAACCCTCACTGAATGGCCGGGAAAGGTACACGCG15980.5311652395894272No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGAACACGCG11570.384579588363559No Hit
GATCCTCCACATATCGAACCCTCAATGACTGGCCGGGAAAGGTACACGCG9990.33206137318513007No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGACAGGTACACGCG9760.3244163165452322No Hit
GATCCTCCACATATCGACCCCTCAATGAATGGCCGGGAAAGGTACACGCG9380.311785353401053No Hit
GATCCTCCACCTATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG8860.29450087751954473No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACCCGCG7120.23666436206988248No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGACGGGAAAGGTACACGCG6150.2044221666755305No Hit
GATCCTCCCCATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG3980.13229271924692868No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGCACACGCG3320.11035473062809126No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGAG3190.10603361165771419No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGGAAGGTACACGCG3020.10038291761952879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)