FastQCFastQC Report
Wed 2 Aug 2023
000000000-GG345_l01_n02_Plate2Col4_mod_third.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GG345_l01_n02_Plate2Col4_mod_third.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83139
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG6496378.13781739015384No Hit
GCTCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG19492.344266830248139No Hit
GATCCTCCACATATCGCACCCTCAATGAATGGCCGGGAAAGGTACACGCG14011.6851297225129No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCTGGGAAAGGTACACGCG7990.9610411479570359No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGCAAGGTACACGCG7130.8575999230204837No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC6740.8106905303166986No Hit
TATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG6290.7565643079661771No Hit
GATCCTCCACATCTCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG5980.7192773547913734No Hit
GATCCTCCACATATCGAACCCTCACTGAATGGCCGGGAAAGGTACACGCG4950.5953884458557356No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAACGGTACACGCG4890.5881716162089994No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGAACACGCG4210.5063808802126559No Hit
GATCCTCCACATATCGACCCCTCAATGAATGGCCGGGAAAGGTACACGCG3720.44744343809764375No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGACAGGTACACGCG2410.28987599081057025No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGACGGGAAAGGTACACGCG2290.2754423315170979No Hit
GATCCTCCACCTATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG2160.25980586728250277No Hit
GATCCTCCACATATCGAACCCTCAATGACTGGCCGGGAAAGGTACACGCG1460.17560952140391392No Hit
GATCCTCCCCATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG1450.1744067164627912No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGCACACGCG1370.16478427693380965No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACCCGCG1320.15877025222819616No Hit
GATCCTCCACATATCGAACCCTCAATGAATTGCCGGGAAAGGTACACGCG1180.1419309830524784No Hit
GATCCTCCACATATCGAACCCTCAATGAATGTCCGGGAAAGGTACACGCG1150.1383225682291103No Hit
GATCCTCCACATATCGAACCCTCAATGAATGCCCGGGAAAGGTACACGCG1070.1287001287001287No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTCCACGCG1060.127497323759006No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAAGTACACGCG1010.12148329905339252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)