FastQCFastQC Report
Wed 2 Aug 2023
000000000-GG345_l01_n02_Plate2Col10_mod_third.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GG345_l01_n02_Plate2Col10_mod_third.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31503
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC2715586.1981398596959No Hit
GCTCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC5031.5966733326984732No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG4651.4760499000095229No Hit
TATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC3381.0729136907596102No Hit
GATCCTCCACATATCGAACCAGCTCTTGATTCAGGTAACCAAAAGCTAAC2760.8761070374250071No Hit
GATCCTCCACATATCGCACCAGATCTTGATTCAGGTAACCAAAAGCTAAC1400.4444021204329746No Hit
GATCCTCCACATATCGAACCCGATCTTGATTCAGGTAACCAAAAGCTAAC1370.4348792178522681No Hit
GATCCTCCACATCTCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC1330.4221820144113259No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAACGCTAAC1310.41583341269085483No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGGAACCAAAAGCTAAC1220.38726470494873505No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAACAGCTAAC710.22537536107672287No Hit
GATCCTCCACATATCGAACCAGATCTTGCTTCAGGTAACCAAAAGCTAAC630.19998095419483858No Hit
GATCCTCCACATATCGACCCAGATCTTGATTCAGGTAACCAAAAGCTAAC530.16823794559248326No Hit
GATCCTCCACATATCGAACCCGCTCTTGATTCAGGTAACCAAAAGCTAAC430.1364949369901279No Hit
GATCCTCCACCTATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC420.1333206361298924No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAAACAAAAGCTAAC390.1237977335491858No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTACC320.10157762752753706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)