FastQCFastQC Report
Tue 25 Jul 2023
000000000-GG345_l01_n02_Plate1Col9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GG345_l01_n02_Plate1Col9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG1435.0No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC37.5No Hit
TCCGGAGACTTATGACAGGATCCTCCCCACATCAAACCCTCCATAAATTG12.5No Hit
TCCGGGGAGGTAGAGACCAGAACCTCCCCAAATAAAACCCTCCCTTACTA12.5No Hit
AGGAAGAGGAGAGAAACCACAAATACCAGACCGTCCCCGTCTGCATAGGA12.5No Hit
CTTAGGCATTTACTCAGGTTTCAGACTTTTATTTCTCCCCATCCTTCCCA12.5No Hit
TCCGTAGTGTCTCGACGGGTTCGGGGACGGCTGCCCCCCCTCACTCACCC12.5No Hit
TGCGGAGAGTAGTGTTGGTTCAGGGCTTGCATAAAATTAGCAATCTACAC12.5No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGAACACGCG12.5No Hit
TCGGAGAGCCTGCGGATCGGAGATGTTATAATAGTACGGACCCTTCACAA12.5No Hit
TCCGAAGAGTCTCGGAGACAGGATCCCCCACCTTTCCCAACATCAATAAA12.5No Hit
ACAGAGGAGGCAGTATTTTGTATTTATATAAGAAACACGGGACTCCACAC12.5No Hit
GATCCTCCACATATCGCACCCTCAATGAATGGCCGGGAAAGGTACACGCG12.5No Hit
ATAGAGGAGTGCTATAGGAAGATCTCCCCACCTATCCTATCTACACATTA12.5No Hit
GCTCCTCCACATATCGAACCCTCACTGAATGGCCGGGAAAGGTACACGCG12.5No Hit
TCAAGAGTGATGCGAATTGGAGAGGAATGTAAGGAACGGGGTCCTCCACA12.5No Hit
GATCCTCCACATATCGAACCCTCAATGACTGGCCGGGAAAGGTACACGCG12.5No Hit
ATTAATTGGTCGGAGATGTGTCAAAAAAGCAGGGTCCTCCCCCATTTCAC12.5No Hit
GATCCTCCACATCTCGAACCCTCAATGAATGACCTGGAAAGGTACACGCG12.5No Hit
GTGTAAAACAGACAAGATACTCCACCCACACACCCCCCAATACCAGGCCG12.5No Hit
ATAAGTTTCTCCACTTGGGTGTGGGCTCCACATATCGCAACCCCAACTTA12.5No Hit
GCTCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG12.5No Hit
TCCGGAGAGTCCCGTTGGTTTGCCGACAGGAACCTCCACAACTCCCACCA12.5No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAACGCTAAC12.5No Hit
AATACTCATTTAGGTTGCTATAACAAAAAATAGCCCCCCCCTCTCGAACA12.5No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)