FastQCFastQC Report
Wed 2 Aug 2023
000000000-GG345_l01_n02_Plate1Col8_mod_third.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GG345_l01_n02_Plate1Col8_mod_third.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170362
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC9666856.74270083704113No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG5810234.10502342071589No Hit
GATCCTCCACATATCGAACCAGCTCTTGATTCAGGTAACCAAAAGCTAAC9540.5599840339981921No Hit
GATCCTCCACATATCGAACCCGATCTTGATTCAGGTAACCAAAAGCTAAC5260.30875429966776624No Hit
GATCCTCCACATATCGCACCAGATCTTGATTCAGGTAACCAAAAGCTAAC5030.2952536363743088No Hit
GATCCTCCACATATCGCACCCTCAATGAATGGCCGGGAAAGGTACACGCG4390.25768657329686195No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGCAAGGTACACGCG4060.23831605639755343No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAACGGTACACGCG3770.22129348094058532No Hit
GATCCTCCACATATCGAACCCTCACTGAATGGCCGGGAAAGGTACACGCG3060.17961752033904274No Hit
TATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC3040.17844354961787254No Hit
GATCCTCCACATCTCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC2860.16787781312734062No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGGAACCAAAAGCTAAC2810.16494288632441506No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAACGCTAAC2570.1508552376703725No Hit
GATCCTCCACATCTCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG2510.14733332550686187No Hit
AATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC2420.1420504572615959No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGAACACGCG2420.1420504572615959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)