FastQCFastQC Report
Mon 31 Jul 2023
000000000-GG345_l01_n02_Plate1Col8_mod.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GG345_l01_n02_Plate1Col8_mod.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169863
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC9651056.81637554970771No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG5800034.14516404396484No Hit
GATCCTCCACATATCGAACCAGCTCTTGATTCAGGTAACCAAAAGCTAAC9460.5569193997515645No Hit
GATCCTCCACATATCGAACCCGATCTTGATTCAGGTAACCAAAAGCTAAC5240.3084838958454755No Hit
GATCCTCCACATATCGCACCAGATCTTGATTCAGGTAACCAAAAGCTAAC5000.29435486244797276No Hit
GATCCTCCACATATCGCACCCTCAATGAATGGCCGGGAAAGGTACACGCG4340.25550002060484034No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGCAAGGTACACGCG4050.23842743858285798No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAACGGTACACGCG3720.21900001766129176No Hit
GATCCTCCACATATCGAACCCTCACTGAATGGCCGGGAAAGGTACACGCG3060.18014517581815934No Hit
TATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC3020.17779033691857554No Hit
GATCCTCCACATCTCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC2850.16778227159534448No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGGAACCAAAAGCTAAC2810.1654274326957607No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAACGCTAAC2550.15012097984846612No Hit
GATCCTCCACATCTCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG2510.14776614094888235No Hit
AATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC2420.14246775342481882No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGAACACGCG2390.140701624250131No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)