FastQCFastQC Report
Wed 2 Aug 2023
000000000-GG345_l01_n02_Plate1Col5_mod_third.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GG345_l01_n02_Plate1Col5_mod_third.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220166
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG10576148.0369357666488No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC9917845.04691914282859No Hit
GATCCTCCACATATCGAACCAGCTCTTGATTCAGGTAACCAAAAGCTAAC7120.32339234940908224No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAACGGTACACGCG5870.2666170071673192No Hit
GATCCTCCACATATCGAACCCGATCTTGATTCAGGTAACCAAAAGCTAAC4120.18713152802885097No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGCAAGGTACACGCG4050.18395210886331223No Hit
GATCCTCCACATATCGAACCCTCACTGAATGGCCGGGAAAGGTACACGCG4020.1825895006495099No Hit
GATCCTCCACATATCGCACCCTCAATGAATGGCCGGGAAAGGTACACGCG3840.17441385136669604No Hit
TATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG2990.13580661864229718No Hit
TATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC2980.1353524159043631No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGAACACGCG2720.12354314471807636No Hit
GATCCTCCACATATCGCACCAGATCTTGATTCAGGTAACCAAAAGCTAAC2400.10900865710418502No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGGAACCAAAAGCTAAC2310.10492083246277809No Hit
GATCCTCCACATCTCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG2280.10355822424897579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)