FastQCFastQC Report
Wed 2 Aug 2023
000000000-GG345_l01_n02_Plate1Col4_mod_third.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GG345_l01_n02_Plate1Col4_mod_third.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229878
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC11373349.47537389397855No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG9799342.62826368769521No Hit
GATCCTCCACATATCGCACCCTCAATGAATGGCCGGGAAAGGTACACGCG9290.40412740671138603No Hit
GATCCTCCACATATCGAACCAGCTCTTGATTCAGGTAACCAAAAGCTAAC8610.3745464985775063No Hit
GATCCTCCACATATCGAACCCGATCTTGATTCAGGTAACCAAAAGCTAAC6040.2627480663656374No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAACGGTACACGCG5660.24621755887905758No Hit
GATCCTCCACATATCGCACCAGATCTTGATTCAGGTAACCAAAAGCTAAC5610.24404249210450762No Hit
GATCCTCCACATATCGAACCCTCACTGAATGGCCGGGAAAGGTACACGCG4470.19445096964476807No Hit
TATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC3840.16704512828543835No Hit
AATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC3770.1640000348010684No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGCAAGGTACACGCG3680.16008491460687843No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGAACACGCG3390.14746952731448854No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGGAACCAAAAGCTAAC3060.1331140866024587No Hit
GATCCTCCACATCTCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG2820.1226737660846188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)