FastQCFastQC Report
Mon 31 Jul 2023
000000000-GG345_l01_n02_Plate1Col4_mod.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GG345_l01_n02_Plate1Col4_mod.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229440
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC11359149.50793235704324No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG9785442.64905857740586No Hit
GATCCTCCACATATCGCACCCTCAATGAATGGCCGGGAAAGGTACACGCG9280.4044630404463041No Hit
GATCCTCCACATATCGAACCAGCTCTTGATTCAGGTAACCAAAAGCTAAC8550.3726464435146444No Hit
GATCCTCCACATATCGAACCCGATCTTGATTCAGGTAACCAAAAGCTAAC6020.26237796373779637No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAACGGTACACGCG5660.24668758716875872No Hit
GATCCTCCACATATCGCACCAGATCTTGATTCAGGTAACCAAAAGCTAAC5610.2445083682008368No Hit
GATCCTCCACATATCGAACCCTCACTGAATGGCCGGGAAAGGTACACGCG4450.1939504881450488No Hit
TATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC3840.16736401673640167No Hit
AATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC3770.164313110181311No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGCAAGGTACACGCG3670.15995467224546722No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGAACACGCG3390.1477510460251046No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGGAACCAAAAGCTAAC3050.1329323570432357No Hit
GATCCTCCACATCTCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG2820.12290794979079497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)