FastQCFastQC Report
Wed 2 Aug 2023
000000000-GG345_l01_n02_Plate1Col10_mod_third.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GG345_l01_n02_Plate1Col10_mod_third.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148761
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG7456550.124024441890015No Hit
GATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC6515843.80045845349251No Hit
GATCCTCCACATATCGAACCAGCTCTTGATTCAGGTAACCAAAAGCTAAC3810.25611551414685296No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAACGGTACACGCG3330.22384899267953295No Hit
GATCCTCCACATATCGCACCCTCAATGAATGGCCGGGAAAGGTACACGCG2820.18956581362050537No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGCAAGGTACACGCG2640.17746586807026035No Hit
GATCCTCCACATATCGAACCCTCACTGAATGGCCGGGAAAGGTACACGCG2310.1552826345614778No Hit
TATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGTACACGCG1990.1337716202499311No Hit
GATCCTCCACATATCGAACCCTCAATGAATGGCCGGGAAAGGAACACGCG1940.13041052426375194No Hit
GATCCTCCACATATCGAACCCGATCTTGATTCAGGTAACCAAAAGCTAAC1760.11831057871350689No Hit
TATCCTCCACATATCGAACCAGATCTTGATTCAGGTAACCAAAAGCTAAC1730.1162939211217994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)