FastQCFastQC Report
Mon 31 Jul 2023
000000000-GG345_l01_n01_Plate2Col9_mod.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GG345_l01_n01_Plate2Col9_mod.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCCGCTTGATCGAGTAAGTCCGTTCCTGAGCAAGCGGGAATTTAACG13.4482758620689653No Hit
AAATTAAGCATTCTCGAGAGTAAGTCCGTTCCTGAGCAAGCGTACATATA13.4482758620689653No Hit
TTTACCAATTGATCGAGTAAGTCCGTTCCTGAGCAAGCGGGAATTTAACG13.4482758620689653No Hit
CGGGCGTCTTGATCGAGTAAGTCCGTTCCTGAGCAAGCGGGAATTTAACG13.4482758620689653No Hit
GTCAGCTCAGTGGAATACCTAGTAAGTCCGTTCCTGAGCAAGCGGGAATT13.4482758620689653No Hit
TTTGCACCTTGATCGAGTAAGTCCGTTCCTGAGCAAGCGTGGATCAGAAG13.4482758620689653No Hit
CCTGGCGATTGATCGAGTAAGTCCGTTCCTGAGCAAGCGGGAATTTAACG13.4482758620689653No Hit
AACGCGTAGCATTGATAGTAAGTCCGTTCCTGAGCAAGCGTCTGCACGAA13.4482758620689653No Hit
TATGCAGATTGATCGAGTAAGTCCGTTCCTGAGCAAGCGGGAATTTAACG13.4482758620689653No Hit
TGTCAATTGGTATCAAGTAAGTCCGTTCCTGAGCAAGCGTTAGCCATGTA13.4482758620689653No Hit
TTCCGACCAGTGGAATACCTAGTAAGTCCGTTCCTGAGCAAGCGGGAATT13.4482758620689653No Hit
TAGATGCTCATTCTCGAGAGTAAGTCCGTTCCTGAGCAAGCGTGGATCAG13.4482758620689653No Hit
TTGGGGACAGTGGAATACCTAGTAAGTCCGTTCCTGAGCAAGCGGGAATT13.4482758620689653No Hit
GGGCCAGATTGATCGAGTAAGTCCGTTCCTGAGCAAGCGGGAATTTAACG13.4482758620689653No Hit
TGGCTTCCTTGATCGAGTAAGTCCGTTCCTGAGCAAGCGGGAATTTAACG13.4482758620689653No Hit
GTAATTGTTTGATCGAGTAAGTCCGTTCCTGAGCAAGCGGGAATTTAACG13.4482758620689653No Hit
ACTCTGCCAGTGGAATACCTAGTAAGTCCGTTCCTGAGCAAGCGGGAATT13.4482758620689653No Hit
TGCGGCAATTGATCGAGTAAGTCCGTTCCTGAGCAAGCGGGAATTTAACG13.4482758620689653No Hit
GTTTATAGTTGATCGAGTAAGTCCGTTCCTGAGCAAGCGGGAATTTAACG13.4482758620689653No Hit
TGCGAGGCTTGATCGAGTAAGTCCGTTCCTGAGCAAGCGGGAATTTAACG13.4482758620689653No Hit
GCGCCCATAGTGGAATACCTAGTAAGTCCGTTCCTGAGCAAGCGGGAATT13.4482758620689653No Hit
TACCGTTCAGTGGAATACCTAGTAAGTCCGTTCCTGAGCAAGCGGGAATT13.4482758620689653No Hit
TTCATCAAGATGTCCTAGTAAGTCCGTTCCTGAGCAAGCGGGAATTTAAC13.4482758620689653No Hit
TTAAAGTAAGTGGAATACCTAGTAAGTCCGTTCCTGAGCAAGCGGGAATT13.4482758620689653No Hit
TCACCATGAGTGGAATACCTAGTAAGTCCGTTCCTGAGCAAGCGGGAATT13.4482758620689653No Hit
TCGAGCTAGCATTGATAGTAAGTCCGTTCCTGAGCAAGCGTCTGCACGAA13.4482758620689653No Hit
CTTCAGGGAGTGGAATACCTAGTAAGTCCGTTCCTGAGCAAGCGGGAATT13.4482758620689653No Hit
TCTTCTCGAGTGGAATACCTAGTAAGTCCGTTCCTGAGCAAGCGTCTGCA13.4482758620689653No Hit
ACGCACGTTTGATCGAGTAAGTCCGTTCCTGAGCAAGCGGGAATTTAACG13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)