FastQCFastQC Report
Sat 2 Oct 2021
000000000-G9BKV_l01_n02_WGS20-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G9BKV_l01_n02_WGS20-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336536
Sequences flagged as poor quality0
Sequence length287
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT11646234.60610454750755No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT211596.287291701333587No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34201.0162360044690613No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8610.25584187130054437No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG7330.21780730739059123No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG6960.20681294126037036No Hit
GGCGATACGACAGCAGTATCTGCTGTCTATGGGCGAGCGCTATGAGCGAC5160.1533268357619987No Hit
GGCGATACGACAGCAGTATCTACTGTCATGGGACTGCACGACAGTCGACT5050.15005824042598712No Hit
GGCGATACGACAGCAGATACTGCTGTCATGGGACTGCACGACAGTCGACT4990.14827537024270807No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4590.1363895690208477No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGTC4360.12955523331827798No Hit
CCGCTATCGACAGCAGTATCTCTATGGGAGAGCGCGCTCGTCGACTATGG4190.12450376779898732No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT3870.11499512682149904No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGTGC3510.10429790572182472No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3390.1007321653552666Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)