FastQCFastQC Report
Sat 2 Oct 2021
000000000-G9BKV_l01_n02_WGS20-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G9BKV_l01_n02_WGS20-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467225
Sequences flagged as poor quality0
Sequence length287
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT69618347.44895977099627No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT51291534.958169333265175No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG136540.9306002828468708Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC36860.2512225459626165Illumina Single End PCR Primer 1 (100% over 50bp)
GCCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT15510.10570975821704237No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)