FastQCFastQC Report
Sat 2 Oct 2021
000000000-G9BKV_l01_n01_WGS20-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G9BKV_l01_n01_WGS20-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336536
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAATGTAATGTGGATGAATACTGCCA5950.17680129317517293No Hit
GAAAATTTGATGTGGATGAATACTGCCA5900.1753155680224404No Hit
ACAACCAGTCATTCCCAACTACGTGCTT5750.17085839256424276No Hit
TGTTTCGAGTTGTGGATGAATACTGCCA5740.17056124753369625No Hit
GTCAAATCAATGTGGATGAATACTGCCA5710.1696698124420567No Hit
CAACAACGTACGAGTGCGTAAGTTGTAT5660.16818408728932419No Hit
TTATTTGTATTGTGGATGAATACTGCCA5590.1661040720754986No Hit
AAATTATTAATGTGGATGAATACTGCCA5590.1661040720754986No Hit
ATCATTATATTGTGGATGAATACTGCCA5500.16342976680058002No Hit
GATTGTATTTTGTGGATGAATACTGCCA5470.1625383317089405No Hit
TAGACCAGTTAGGCTTATTCCTCCATTA5470.1625383317089405No Hit
ATCTGCTATATGTGGATGAATACTGCCA5410.16075546152566145No Hit
AAGCCTATTCTGTGGATGAATACTGCCA5230.15540685097582427No Hit
ATGAAAAGGCTGTGGATGAATACTGCCA5230.15540685097582427No Hit
ATGAATATATTGTGGATGAATACTGCCA5210.15481256091473128No Hit
GGGCTACAGCTAATGAAGCCACGTCTAG5210.15481256091473128No Hit
CATGGTAAGCTGGCTCATAACCTCGGAT5210.15481256091473128No Hit
CGGGTGCCAAGGAACTCCAGTCACCGAC5170.15362398079254522RNA PCR Primer, Index 4 (96% over 28bp)
AGACTCATCTATCCATGACATTTGCCGG5150.1530296907314522No Hit
ATTCGTGGGTTGTGGATGAATACTGCCA5140.15273254570090572No Hit
TTGACCCGATTGTGGATGAATACTGCCA5090.15124682054817315No Hit
ATCTTATACATGTGGATGAATACTGCCA5070.15065253048708013No Hit
TGTATTTGTATGTGGATGAATACTGCCA5040.1497610953954406No Hit
TATCCGAATTTGTGGATGAATACTGCCA5030.1494639503648941No Hit
TTCGTTGAGCTGTGGATGAATACTGCCA5000.14857251527325455No Hit
ATCTCGTGGATGTGGATGAATACTGCCA5000.14857251527325455No Hit
CCACTCGTGTTGTGGATGAATACTGCCA5000.14857251527325455No Hit
CTTATTTCTTTGTGGATGAATACTGCCA4940.14678964508997552No Hit
ATTGTTCTGATGTGGATGAATACTGCCA4920.14619535502888248No Hit
CGTTGCTAAATGTGGATGAATACTGCCA4920.14619535502888248No Hit
AAGTAGGTATTGTGGATGAATACTGCCA4880.14500677490669647No Hit
GTTGTCTGTTTGTGGATGAATACTGCCA4880.14500677490669647No Hit
GAATATGTTTTGTGGATGAATACTGCCA4880.14500677490669647No Hit
ACGTAGTTATTGTGGATGAATACTGCCA4880.14500677490669647No Hit
TGAGGGACTATGTGGATGAATACTGCCA4850.14411533981505692No Hit
AAGTGGGGCCTGTGGATGAATACTGCCA4810.1429267596928709No Hit
GACACGCAGCCTTCTCGAGAGCACGAGG4810.1429267596928709No Hit
CGTGGTCAGATGTGGATGAATACTGCCA4780.14203532460123136No Hit
TGAGTCACAACCGTATAGTTCCTCCCGT4760.14144103454013834No Hit
GACATTTTACTGTGGATGAATACTGCCA4750.14114388950959184No Hit
CCTTCACTTTTGTGGATGAATACTGCCA4750.14114388950959184No Hit
GAGTCATTGTTGTGGATGAATACTGCCA4730.14054959944849882No Hit
CAGTTCATTTTGTGGATGAATACTGCCA4720.1402524544179523No Hit
ATAGTGTGATTGTGGATGAATACTGCCA4710.1399553093874058No Hit
TATCGCCTCCATAGACGGACGCGTTGAC4690.13936101932631278No Hit
ATCTATGTGCTGTGGATGAATACTGCCA4640.13787529417358024No Hit
ATCGTCTTGTTGTGGATGAATACTGCCA4620.13728100411248723No Hit
AAGATCGGTTTGTGGATGAATACTGCCA4620.13728100411248723No Hit
GTGGACTTGGTGTGGATGAATACTGCCA4610.1369838590819407No Hit
CAATGACTCTCTAGGATGATTGGCGATT4590.1363895690208477No Hit
TGACACCTTCTGTGGATGAATACTGCCA4590.1363895690208477No Hit
TTAGTATGTTTGTGGATGAATACTGCCA4560.13549813392920818No Hit
CGTCAAATCAAAGGTACATTTAATTACA4560.13549813392920818No Hit
TTGGTACGAATGTGGATGAATACTGCCA4530.13460669883756862No Hit
CCGTTACAATTGTGGATGAATACTGCCA4520.13430955380702214No Hit
TGCAGGCTCGACGATTGTAACGATGACT4480.1331209736848361No Hit
TTCAGGATCCAGCCTTGTTATTGTAACG4480.1331209736848361No Hit
TTGTTCAGTGATGGCAACTTTTAATAAA4470.13282382865428957No Hit
TTTTAAAGGATGTGGATGAATACTGCCA4440.13193239356265007No Hit
CGTCAGATGCTGTGGATGAATACTGCCA4430.13163524853210357No Hit
TATCTTACTCTGTGGATGAATACTGCCA4430.13163524853210357No Hit
GTAGAAAGAATGTGGATGAATACTGCCA4420.13133810350155706No Hit
GTAACCAAGTCGGGATCTACACATATTG4420.13133810350155706No Hit
GGGCCAAGTTTGTGGATGAATACTGCCA4410.13104095847101052No Hit
CACGTGGTCCTGTACCAGCGTTATAGCT4400.130743813440464No Hit
GCAGGGTGCTTGTGGATGAATACTGCCA4390.1304466684099175No Hit
CCCAACTGTAGCGTCCACCAAATCCCTT4370.1298523783488245No Hit
CAACCTCAGCGTCTCGGTCTGTTGCATT4340.12896094325718496No Hit
AGGAAATTTGTGTGGATGAATACTGCCA4330.12866379822663845No Hit
ATCCACCCAGAGAGGGTTTTCTATACGC4320.12836665319609195No Hit
TGAATCGGTGGATGACATCGGCCTTTAG4320.12836665319609195No Hit
CATAAGCAGCTGTACTTTTCAAGTGTCG4310.12806950816554544No Hit
ATTGGTCATTTGTGGATGAATACTGCCA4280.1271780730739059No Hit
TAGACTGAGTGGTTGGAGCATATTCCAT4280.1271780730739059No Hit
AGTCAAGGTTTGTGGATGAATACTGCCA4260.1265837830128129No Hit
TCTAACTCAGCACACCGAATATAGTCAG4250.1262866379822664No Hit
GACCAATAGGCTTCCGCTGTTGTTGAGT4240.12598949295171988No Hit
GCTTCCCCTTTGTGGATGAATACTGCCA4240.12598949295171988No Hit
ACTTTCATCATGAGTCCCGATAGCCCAA4240.12598949295171988No Hit
TTGTGTTCGGTGTGGATGAATACTGCCA4230.12569234792117337No Hit
TAGCGCTATGTGTGGATGAATACTGCCA4210.12509805786008035No Hit
TGTTCCGAGGGATCTGGATCAGGTGTAC4210.12509805786008035No Hit
TGGTTGTTCTTGTGGATGAATACTGCCA4190.12450376779898732No Hit
TGCAGATGTAGTCCTAAACATTCGGTAT4150.12331518767680129No Hit
ATTCTCTATCTGTGGATGAATACTGCCA4130.12272089761570827No Hit
ACGGAAGCAGCGGATATTACACGGCAGC4130.12272089761570827No Hit
GGCTCTTTGCTGTGGATGAATACTGCCA4120.12242375258516176No Hit
TGCATGAAGCGGATCAAGGGGAACTTCA4120.12242375258516176No Hit
TTACAGGGTCAGACTTACCCAGCTGTTA4100.12182946252406876No Hit
TAGGATAGTCTGTGGATGAATACTGCCA4060.12064088240188271No Hit
TCCGAAATCAAACGTCCCGTACCCCAAT4040.1200465923407897No Hit
AGAATTAGTATGTGGATGAATACTGCCA4040.1200465923407897No Hit
GAATCGTAGGCTAGCACTTGCTTCTGTT4000.11885801221860365No Hit
ATTCTACGTTAACAGACTGTTATTTAGC4000.11885801221860365No Hit
TTTATGGTTGTGTGGATGAATACTGCCA3910.11618370694368507No Hit
AAATGGAACTTGTGGATGAATACTGCCA3910.11618370694368507No Hit
ATTGCGTTCCTGTGGATGAATACTGCCA3910.11618370694368507No Hit
AGTACAAGCATGTGGATGAATACTGCCA3900.11588656191313858No Hit
AGGCCGCTGTTGTGGATGAATACTGCCA3900.11588656191313858No Hit
TCCTTCTGTACGTAGGGTTCATCCAGTA3890.11558941688259204No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAG3850.11440083676040602RNA PCR Primer, Index 1 (100% over 28bp)
TTTCATGCATGGCTATATTGGGACTCAC3850.11440083676040602No Hit
GGACAGGTCATGTGGATGAATACTGCCA3840.11410369172985951No Hit
TGTTAATGTATGTGGATGAATACTGCCA3830.113806546699313No Hit
CTTCCTTCAGATAAACAACCCGCGTTAT3830.113806546699313No Hit
GGTGGCCTAATGTGGATGAATACTGCCA3810.11321225663821997No Hit
AGGAAATTCCGTGGGTGCAACTAGTGAA3810.11321225663821997No Hit
CTTTATGTAGTGTGGATGAATACTGCCA3810.11321225663821997No Hit
CCTATCGGTACTCAACAACCTCTGGGGG3790.11261796657712697No Hit
AGGGTCCAGCAAATCATCTTATCTAAAG3780.11232082154658046No Hit
CGGCAGTCAATGTCACCATCCTGCAGTG3770.11202367651603395No Hit
ATTATCTTACTGTGGATGAATACTGCCA3760.11172653148548743No Hit
ATCAGGCAGATGTGGATGAATACTGCCA3750.11142938645494092No Hit
CTGATCTTATTGTGGATGAATACTGCCA3710.1102408063327549No Hit
AGGCATTTCTTCTTCCTCCATAAAAAGG3700.1099436613022084No Hit
GGGACCTTCGAGTCCGATTCGTACAGGT3680.10934937124111535No Hit
GGAGATGAGCTTACGTTTTCGGTTTCAT3560.10578363087455725No Hit
CGGGTAAAAGTGTGGATGAATACTGCCA3550.10548648584401074No Hit
ATCGGCGTGGTGTGGATGAATACTGCCA3540.10518934081346423No Hit
CGAAGTTAGGTTCTACTACCAACCGCGG3530.10489219578291772No Hit
AACCATGCACAGTATCAAAATCTTAAGC3520.10459505075237122No Hit
CCCCGCGCCTTGTGGATGAATACTGCCA3490.10370361566073168No Hit
TGAACGTCACAGTCGCCTCACCCTTTCA3470.10310932559963867No Hit
TTCGAGCAGATGTGGATGAATACTGCCA3470.10310932559963867No Hit
GATGGTTGTTTGTGGATGAATACTGCCA3460.10281218056909217No Hit
TGTGAGGGTTTGTGGATGAATACTGCCA3450.10251503553854566No Hit
CTTTCAAAGCATTGTCGGATTCCAAGAC3450.10251503553854566No Hit
CAATCGAAGAATTGTGGGAGTTTACGGA3440.10221789050799915No Hit
ATCCTATTCGCCACTTCCACTCTCTCGT3410.10132645541635961No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)