FastQCFastQC Report
Sat 2 Oct 2021
000000000-G9BKV_l01_n01_WGS20-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G9BKV_l01_n01_WGS20-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467225
Sequences flagged as poor quality0
Sequence length28
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTGCCAAGGAACTCCAGTCACGTGT305292.0807306309529894RNA PCR Primer, Index 15 (96% over 28bp)
GGAATTCTCGGGTGCCAAGGAACTCCAG158661.0813610727734329RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCA44030.30009030653103647RNA PCR Primer, Index 1 (100% over 28bp)
CTCGGGTGCCAAGGAACTCCAGTCACGT32170.21925744176932646RNA PCR Primer, Index 1 (96% over 28bp)
TCTCGGGTGCCAAGGAACTCCAGTCACG19990.1362435890882448RNA PCR Primer, Index 6 (100% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)