FastQCFastQC Report
Sat 18 Dec 2021
000000000-G992J_l01_n02_WGS21-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G992J_l01_n02_WGS21-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126836
Sequences flagged as poor quality0
Sequence length287
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG116189.159859976662778No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT60124.739979185720142No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG43113.3988772903592044Illumina Single End PCR Primer 1 (100% over 50bp)
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT24551.935570342804882No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC14801.1668611435239207Illumina Single End PCR Primer 1 (100% over 50bp)
TACAACTTGATGTTATGGATTTAGTTGATAAGAGATACTCCATTAAATTT6250.4927623072313854No Hit
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC5430.4281118925226276Illumina Single End PCR Primer 1 (100% over 49bp)
AATCAGTATTGAATTTTTGAATAAATCATAAATTCATATACAAAGAAAAT5400.42574663344791697No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5230.4123434986912233No Hit
GACAGCAGTATCTATGGAGTCGCGAGCGCTATGAGCGACTATGGATCGAC4890.38553722917783595No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC4580.36109621873915926Illumina Single End PCR Primer 1 (100% over 49bp)
AAGCAAGTTATACCCAATCCCTTGTATCATTTAGCTTAGTTTCTACGTTT4170.3287710113847803No Hit
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC4060.32009839477750796Illumina Single End PCR Primer 1 (100% over 49bp)
AAAAACGCATTTTTAGACTTCTATTGTAAGCGCTTTTTGTTTGCACGAGA4050.3193099750859377No Hit
GCTGACGATCGGGTGAGCGGAAGTGAGATATATGTACAGTGGCTCGCAAC3760.29644580403040144No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCATGG3170.2499290422277587No Hit
GCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG3070.24204484531205653Illumina Single End PCR Primer 1 (100% over 48bp)
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGATGGGTCAGC3050.2404680059289161No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGCTATGGGAGAGCATAGACAT3020.23810274685420546No Hit
GACAGCAGTATCATCCTATCCATCCATCCATCCTATGGAGTCGCGAGCGC3010.23731432716263523No Hit
GACAGCAGTATCATCGAGTCGTCAGCAGTACTACTGACGATGGAGTCGAC2980.23494906808792454No Hit
GACAGCAGTATCATCGAGTATGGAGTCGACTACAGTCGCTATGGAGTCGA2970.23416064839635434No Hit
GACAGCCTATGGAGTCGAGAGCGCCTATGGAGTCGTCAGCAGTACTACTG2910.22943013024693304No Hit
GACAGCAGTATCATCGAGTCCAGTATGGAGTCGACTACAGTCGCTACGAG2820.22233435302280108No Hit
GACAGCAGTATCATCGATGGATGGAGTCGAGAGCGCGCTCGTCGTATGGA2820.22233435302280108No Hit
GACAGCAGTATCATAGGTATCCATACTATGGATACCATACCATAGTATCT2760.21760383487337978No Hit
GACAGCAGTATCATCTATGGAGTCGCGAGCGCTATGAGCTATGGATCGAC2730.21523857579866915No Hit
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC2700.21287331672395848Illumina Single End PCR Primer 1 (100% over 49bp)
GACAGCAGTAGACAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACTA2630.20735437888296698No Hit
GACAGCAGTATGGAGTCGACTACAGTCGCTACGACTATGGAGTCGACAGA2610.20577753949982655No Hit
GACAGCAGTATCATCGATGTCTATGCGCTACGACTATGGAGTCGACAGAG2490.19631650320098396No Hit
GACAGCAGTATCATCGAGTATGGAGTCGAGAGCGCGCTATGGAGTCGTCA2450.1931628244347031No Hit
GACAGCAGTATCATCGCTATGGAGTCGTCAGCAGTACTACTGAAGTATGG2450.1931628244347031No Hit
GACAGCAGTATCATCTATGGAGTCGACAGAGATATCATGCAGTATGGAGT2430.19158598505156266No Hit
GACAGCAGTATCCATGGGTATGGAGTCGACAGCAGTATCTGCTGTCATGG2410.19000914566842222No Hit
GACAGCAGTATCATCGAGATGGAGTACTACTGCAGTACTACTGACGATGG2330.1837017881358605No Hit
GACAGCAGTATCATCGACACTATGGAGTCGACAGAGATATCTATGGAGTC2310.18212494875272006No Hit
CTATGGAGTCCTATGGAGTCGTCAGCAGTACTACTGACGATGGAGTCGAC2230.17581759122015833No Hit
TGTTGAGAAGCAGCATTCCATTGTAATATTACTTACTAGTGTGTTCGCTA2220.1750291715285881No Hit
GACAGCAGTATCATCCATGACAGCAGATACTGCTGTCGACTCCATACTGC2210.1742407518370179No Hit
GACAGCAGTATCTGGAGTCGAGAGCGCCTATGGAGTCGTCAACTATGGAT2170.17108707307073703No Hit
GACAGCAGTATCATCGATGGAGTCGACAGCAGTGTGTGAGTCGAGAGCAT2170.17108707307073703No Hit
GACAGCAGTATCATCGATGATATGGAGGATATGGATATGGATAGTATGGA2160.1702986533791668No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCCTATGGAGTCGTCAGCAGTA2130.16793339430445617No Hit
AAGCAATACGGCGCACCGGTGGAGCGGTTGAAAGATTAAGGCCACCGGGT2030.16004919738875398No Hit
GACAGCAGTATCATCGATGTCTATGCGAGTATGGAGTATGGAGTCGACTA2000.15768393831404331No Hit
GACAGCAGTATCATCGCTATGGAGTGTATGGAGTCGACTACAGTCGCTAC1990.1568955186224731No Hit
GACAGCAGTATCATCGACTCATCATATGGATGTAGTCGCTATGGAGTCGA1960.15453025954776245No Hit
GACAGCAGTATCATCGATATCTATGGAGTCGAGAGCTATGGATCGACAAA1920.15137658078148158No Hit
GACACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGT1920.15137658078148158No Hit
GACAGCAGTATCATCGCGCAGCTATGGATCGCAAGGTGGCAGCTATGGAG1860.1466460626320603No Hit
GACAGCAGTATCATCGCTATGGAGTCGCGAGCGCCTATGGATCGACAAAT1840.14506922324891985No Hit
GACAGCAGTATCATCTATGGAGTCGAGAGAGTATGGAGTCGACTACAGTC1830.14428080355734965No Hit
TCTATGGAGTCGCGAGCGCTATGAGCGACTATGGATCGACAAATGGCAGT1820.14349238386577942No Hit
GACAGCAGTATCATCGAGTCGAGAGCGCGCCTATGGAGTCGTCAGCAGTA1820.14349238386577942No Hit
GACAGCAGTATCATCGTATGGAGTCGACAGCAGTATCTGCTGTCATGGAG1820.14349238386577942No Hit
GACTCCAGTACTACTGACGATGGAGTCGAGAGCATAGTATGGAGTCGACT1810.14270396417420922No Hit
GCTATGGAGTCGAGAGCGCGCTCGTCGAGTATGGAGTCGACTACAGTCGC1790.1411271247910688No Hit
GACAGCAGTATCATCGAGAGCGCGCTCGTGACTATGGATCGACAAATGGC1790.1411271247910688No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCGCGCTCGTCTGGACAGACAT1790.1411271247910688No Hit
GACAGCAGCTATGGAGTCGCGAGCGCTATGAGCGACTATGGATCGACAAA1760.13876186571635812No Hit
GACAGCAGTATCATCGGAGTCGAGAGCATAGACATCGACTGGAGTCGACT1750.13797344602478792No Hit
GGCGATACCTATGGGCGAGCGCTATGAGCGACTAAGTATGGGACTACAGT1740.1371850263332177No Hit
GACAGCAGTATCATCGTGATACTATGGAGTCGCGAGCGCTATGAGCGACT1710.13481976725850706No Hit
GACAGCAGTATCGATGGATCGACAAATGGCAGTATTCATCCACAATCACC1700.13403134756693683No Hit
GACAGCAGTATCATCGATGATACTCTATAGATGATCATCTCTATCTATGG1690.13324292787536662No Hit
GACAGCAGTATCATCGAAGTATGGAGTCGACTACAGTCGCTACGACTATG1680.1324545081837964No Hit
GACAGCAGTATCATCGGAGTCGACAGCAGTGTGTGAGTCGAGAGCATAGA1640.12930082941751553No Hit
AAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG1640.12930082941751553Illumina Single End PCR Primer 1 (100% over 48bp)
GACAGCAGTATCATCGCTGCAGTATCCATCATTCCTATGGAGTCGCGAGC1630.12851240972594533No Hit
GACAGCAGTATCATCGAATGATGGATCGAATTGTCTATGGATCGATTCGA1620.1277239900343751No Hit
TCGGAGTCCATGGAGTCGCGAGCGCTATGGATCGACAAATGGCAGTATTC1600.12614715065123466No Hit
GACAGCAGTATCATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT1590.12535873095966446No Hit
GACAGCAGTATCATCGATACGGAGTCGCGAGCGCTATGATCGACAAATGG1580.12457031126809424No Hit
GACAGCAGTATCATCGATGATATGGAGTCGAGAGCGCGCTCGTATGGAGT1570.12378189157652403No Hit
GACAGCAGTATCTGCTGTCATGGAGTCGCGAGCGCTATGAGCTATGGATC1570.12378189157652403No Hit
GACAGCAGTATCATCATCGTATGGAGTCGACTACTATGGAGTCGACAGAG1550.1222050521933836No Hit
GACAGCAGTATCATCGAGAGTCGAGAGCGCGCTATGGAGTCGACAGAGAT1530.12062821281024316No Hit
GACAGCAGTATCATTCTTAAATTTCCACGTTAATTAAGAAAAATTAAGTA1530.12062821281024316No Hit
GACAGCAGTATCATCGATGGAGTCGCGAGCGCTATGAGCGACTATGGATC1520.11983979311867295No Hit
GACAGCAGTATATGGAGTCGTCAGCAGTACTACTGACGATGGAGTCGACA1520.11983979311867295No Hit
GACAGCAGTATCATCATCAGTATGGAGTCGAGAGCGCTATGAGCGACTAT1510.11905137342710273No Hit
GACAGCAGTATCATCTGGAGTCGACTCCAGTACTGGAGTCGAGAGCGCGC1510.11905137342710273No Hit
GACAGCAGTATCATCGAGTATGGATCGACAAATGGCAGTATTCATCCACA1500.11826295373553249No Hit
GACAGCAGTATCATCGGATAATCGACGAGCGCGCTCTCGCTATGGAGTCG1490.11747453404396227No Hit
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG1470.11589769466082184Illumina Single End PCR Primer 1 (100% over 48bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG1470.11589769466082184Illumina Single End PCR Primer 1 (100% over 48bp)
GACAGCAGTATCATCTATGGAGTCGAGAGCGCGCTCGTCGATGGAGTCGA1470.11589769466082184No Hit
GACAGCAGTATCATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG1460.11510927496925162No Hit
GACAGCAGTATCATCGACGAGCGCGCTCTCGACTATGGAGTCGTCAGCAG1410.11116717651140054No Hit
GACAGCAGTATCATCGATCGACAAATGGCAGTATTCATCCACAATGACAA1410.11116717651140054No Hit
GACAGCAGTATCATCATGGAGTCGCGAGCGCTATGAGCTATGGATCGACA1400.11037875681983032No Hit
GACAGCAGTATCATCCTATGGAGTAGTATGGAGTCGACAGCAGTATCTGC1390.1095903371282601No Hit
GACAGCAGTATCATCGAGTCGAGTCGATGGAGTCGACTATGGAGTCGTCA1380.10880191743668989No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCATAGACATATAGAGCATATG1380.10880191743668989No Hit
GACAGCAGTATCATCGAGTCGACAAATGGCAGTATTCATCCACATCATCC1370.10801349774511967No Hit
GACAGCAGTATCATCTATGGAGTCGAGAGCGCGCTCGTGAGTCGAGAGCA1370.10801349774511967No Hit
GACAGCAGTATCATCGATGGACTCGACTATGGAGTCGACAGAGATATCAT1360.10722507805354946No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGATTGTAGATCTCGGTGGTCGCCG1360.10722507805354946Illumina Single End PCR Primer 1 (98% over 50bp)
GACAGCAGTATCAAGGTCCATAGGTCCATAGGTCCTATGGAGTCGTCAGC1350.10643665836197924No Hit
GACAGCAGTATCATCGACAAATGGCAGTATTCATCCACACGATAACGGAA1350.10643665836197924No Hit
GTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG1340.10564823867040903Illumina Single End PCR Primer 1 (100% over 48bp)
ACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG1330.10485981897883881Illumina Single End PCR Primer 1 (100% over 48bp)
GACAGCAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAG1300.10249455990412816No Hit
GACACTATGGAGTCGCGAGCGCTATGAGCGACTATGGATCGACAAATGGC1290.10170614021255794No Hit
GACAGCAGTATCATCGAGTATGGAGTCGACTACAGTCGCTACGACTATGG1290.10170614021255794No Hit
GACAGCAGTATCATCATCAGCATATGGAGTCGAGAGCATAGACATCCTAT1290.10170614021255794No Hit
GACAGCAGTATCGTCGACTATGGAGTCGCGATGGATCGACAAATGGCAGT1290.10170614021255794No Hit
GACAGCAGTATCATCGTCGATGATGGATGGATGGATCGATGGAGTCGAGA1280.10091772052098773No Hit
GACAGCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGC1270.10012930082941751No Hit
GACAGCAGTATCATCTATCTATGGAGTCGAGAGCGCGCTCCTATGGAGTC1270.10012930082941751No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)