FastQCFastQC Report
Sat 18 Dec 2021
000000000-G992J_l01_n01_WGS21-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G992J_l01_n01_WGS21-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476135
Sequences flagged as poor quality0
Sequence length28
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTGCCAAGGAACTCCAGTCACTATA49481.0392010669242966RNA PCR Primer, Index 43 (96% over 28bp)
GGAATTCTCGGGTGCCAAGGAACTCCAG17630.37027313682043955RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCA5780.12139414241759165RNA PCR Primer, Index 1 (100% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)