FastQCFastQC Report
Sat 18 Dec 2021
000000000-G992J_l01_n01_WGS21-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G992J_l01_n01_WGS21-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348450
Sequences flagged as poor quality0
Sequence length28
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTGCCAAGGAACTCCAGTCACCTCG13080.37537666810159276RNA PCR Primer, Index 12 (96% over 28bp)
AATACCAAAATGTGGATGAATACTGCCA8800.25254699382981777No Hit
ACCTTGTGACTGTGGATGAATACTGCCA8580.2462333189840723No Hit
TGTGTTTACTTGTGGATGAATACTGCCA8240.23647582149519303No Hit
TAGGTAGTGGTGTGGATGAATACTGCCA8130.23331898407232027No Hit
TGCATTTAGGTGTGGATGAATACTGCCA7520.21581288563638973No Hit
TGTATAAATATGTGGATGAATACTGCCA7470.21437795953508396No Hit
TTCAATCCTTTGTGGATGAATACTGCCA7430.21323001865403932No Hit
GGGGGCCTGATGTGGATGAATACTGCCA6820.19572392021810878No Hit
AACAATTATTTGTGGATGAATACTGCCA6810.1954369349978476No Hit
TGTTCTACACCTTCGTTGAATTCTTGTT6790.1948629645573253No Hit
ATTTTAACGCTGTGGATGAATACTGCCA6670.1914191419141914No Hit
CGTTGGTTGGTGTGGATGAATACTGCCA6440.1848184818481848No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAG5790.16616444253120963RNA PCR Primer, Index 1 (100% over 28bp)
TTGAGCCCCATGTGGATGAATACTGCCA5570.15985076768546422No Hit
TTCTAACCAGGATCTTTCGTTTCCAGTT5470.15698091548285265No Hit
CAACAGTTCTTGTGGATGAATACTGCCA5280.15152819629789066No Hit
TCTAACTCAACAGCTTCCACTTGTCTCA5120.14693643277371216No Hit
GCTACAACATCGCTGGTTAATCGAATTA4890.14033577270770556No Hit
TCCTAATCATTCTTCAACACGTTCAGAG4860.13947481704692208No Hit
GCTGGGTTCATGCCGGCTTTAATTTGGG4760.13660496484431053No Hit
AATACATCTATGTGGATGAATACTGCCA4720.1354570239632659No Hit
TTAAAGTGTTTGTGGATGAATACTGCCA4340.12455158559334194No Hit
GGTGAAGTCGGCTCTTAAACCTAAGGTC4320.12397761515281965No Hit
GTCTACAATCTGTGGATGAATACTGCCA4290.12311665949203615No Hit
TATAAACCGATGTGGATGAATACTGCCA4260.1222557038312527No Hit
GGCAATTAGTTGTGGATGAATACTGCCA4250.12196871861099154No Hit
GTCCGTTGCTTGTGGATGAATACTGCCA4230.12139474817046922No Hit
AGATAGACAATGTGGATGAATACTGCCA4230.12139474817046922No Hit
AAATGGAGTAAGTTGAGGTGACAATGGT4220.12110776295020807No Hit
AATTGAGGAATGTGGATGAATACTGCCA4200.12053379250968575No Hit
TGCTTATATGTGTGGATGAATACTGCCA4190.12024680728942459No Hit
TGTTACCACATGTGGATGAATACTGCCA4140.1188118811881188No Hit
TCTAGTAGCTTGTGGATGAATACTGCCA4120.11823791074759651No Hit
AGTTGTTTGCTGTGGATGAATACTGCCA4100.1176639403070742No Hit
ATGGGAGCACGTGAGAGATGCCCTCAAT4080.11708996986655186No Hit
AAGATTTGTTTGTGGATGAATACTGCCA4070.11680298464629073No Hit
GCTAATAACTTGTGGATGAATACTGCCA4070.11680298464629073No Hit
TGTACTAATTTGTGGATGAATACTGCCA4040.11594202898550725No Hit
TTATATTTAATGTGGATGAATACTGCCA4040.11594202898550725No Hit
TATAGCCATATGTGGATGAATACTGCCA4020.11536805854498494No Hit
AATAGTTTGATGTGGATGAATACTGCCA4020.11536805854498494No Hit
ACTCCCAAGTGCAAATGCGCCAACCTTC4020.11536805854498494No Hit
TATGTGCACTTGTGGATGAATACTGCCA4010.11508107332472378No Hit
CAATCAATTATGTGGATGAATACTGCCA3990.11450710288420146No Hit
ACTAAGCTATTGTGGATGAATACTGCCA3970.11393313244367916No Hit
ATGAATCTTCTGTGGATGAATACTGCCA3940.11307217678289568No Hit
GTTGGGGTTCTGTGGATGAATACTGCCA3930.11278519156263452No Hit
TCATAACAAATGTGGATGAATACTGCCA3910.11221122112211222No Hit
CTTATTATTCTGTGGATGAATACTGCCA3910.11221122112211222No Hit
GAGACTTGTAGGGTACTAATGATATTGT3900.11192423590185105No Hit
TGATAGTATGTGTGGATGAATACTGCCA3880.11135026546132873No Hit
AAATTTCTCCTGTGGATGAATACTGCCA3880.11135026546132873No Hit
TCAGAACCTATGTGGATGAATACTGCCA3870.1110632802410676No Hit
CTATAGTTCTTGTGGATGAATACTGCCA3870.1110632802410676No Hit
CTAATTCTGCTGTGGATGAATACTGCCA3870.1110632802410676No Hit
AATGTATTTGTGTGGATGAATACTGCCA3870.1110632802410676No Hit
CCTTAATGGCTGTGGATGAATACTGCCA3860.11077629502080644No Hit
TGGTATATTATGTGGATGAATACTGCCA3860.11077629502080644No Hit
CTGTAAATAATGTGGATGAATACTGCCA3850.11048930980054528No Hit
GAGGTAGCGTTGTGGATGAATACTGCCA3840.11020232458028412No Hit
TATTATCGTCTGTGGATGAATACTGCCA3840.11020232458028412No Hit
AATTAAATTGTGTGGATGAATACTGCCA3840.11020232458028412No Hit
CCATAAGCATGTCTAGGCTTTCTAGTTA3840.11020232458028412No Hit
GATGGAGAGCGCCTCACAGTTAAGATTG3820.10962835413976181No Hit
ATGCGATTGTTGTGGATGAATACTGCCA3810.10934136891950065No Hit
ATTGCGACTTTGTGGATGAATACTGCCA3810.10934136891950065No Hit
TATTCCACAGTGTGGATGAATACTGCCA3800.10905438369923949No Hit
GAACTGTCACATATCGTTTAATCTTAAC3780.10848041325871717No Hit
CGAGTTTTGTTGTGGATGAATACTGCCA3780.10848041325871717No Hit
GGTTCTGGTGTGTGGATGAATACTGCCA3780.10848041325871717No Hit
CAACAAGTACTGTGGATGAATACTGCCA3770.10819342803845602No Hit
ACAACTCGTATGTGGATGAATACTGCCA3770.10819342803845602No Hit
AGGTTGTCCGTGTGGATGAATACTGCCA3770.10819342803845602No Hit
TGAGGGAATTTGTGGATGAATACTGCCA3750.1076194575979337No Hit
AAGTTCGGTATGGTTCCAAGTGCGTTCT3730.10704548715741138No Hit
TGAGGGTATCTGTGGATGAATACTGCCA3710.10647151671688909No Hit
TCCTTTTAATTGTGGATGAATACTGCCA3710.10647151671688909No Hit
CTCCGCATTTTGTGGATGAATACTGCCA3700.10618453149662793No Hit
GAAACCTGTTGGTGTTAAATTATATCCG3690.10589754627636676No Hit
CTTGTCTATATGTGGATGAATACTGCCA3680.1056105610561056No Hit
ATGGATGTGATGTGGATGAATACTGCCA3680.1056105610561056No Hit
TCGAATGTGCTGTGGATGAATACTGCCA3670.10532357583584447No Hit
GAAATGGCCTTGTGGATGAATACTGCCA3650.10474960539532215No Hit
TGCACAAAAGTGTGGATGAATACTGCCA3650.10474960539532215No Hit
TTCTAATAAGTGTGGATGAATACTGCCA3640.10446262017506099No Hit
TAGCAATGGTTGTGGATGAATACTGCCA3620.10388864973453868No Hit
ACGATCGGTTTGTGGATGAATACTGCCA3620.10388864973453868No Hit
TATTCGTATGTGTGGATGAATACTGCCA3610.10360166451427752No Hit
AGATTTACCTTGTGGATGAATACTGCCA3610.10360166451427752No Hit
ATTAAATATGTGTGGATGAATACTGCCA3610.10360166451427752No Hit
TTTCGGGCTTTGTGGATGAATACTGCCA3600.10331467929401636No Hit
ACTGTTATGTTGTGGATGAATACTGCCA3590.1030276940737552No Hit
CGATTAATAGTGTGGATGAATACTGCCA3570.1024537236332329No Hit
TCATTCTGAATGTGGATGAATACTGCCA3550.10187975319271057No Hit
GGGATTTTACTGTGGATGAATACTGCCA3550.10187975319271057No Hit
TGACAGTGTATGTGGATGAATACTGCCA3540.10159276797244941No Hit
TGTATTGTTATGTGGATGAATACTGCCA3540.10159276797244941No Hit
TAATAAGTAATGTGGATGAATACTGCCA3540.10159276797244941No Hit
TTGGGCGTGGTGTGGATGAATACTGCCA3510.10073181231166596No Hit
GTTACAGATCTGTGGATGAATACTGCCA3510.10073181231166596No Hit
TTCCACCGGCTGTGGATGAATACTGCCA3510.10073181231166596No Hit
ATCTTTCCCATGTGGATGAATACTGCCA3500.10044482709140479No Hit
ATCGGAGATATGTGGATGAATACTGCCA3490.10015784187114363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)