FastQCFastQC Report
Thu 20 Oct 2022
000000000-G98TK_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G98TK_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6481489
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGCGT4521926.976668478493137No Hit
CTAGTATG4132536.37589603253203No Hit
GTCTAGTG3993976.162117994800269No Hit
CGATCTAC3520105.43100512860548No Hit
TGCGTCAC3158884.8736949179424665No Hit
ACGCGTGA3030004.674851720029148No Hit
AAGCAGCA2904944.4819022295648425No Hit
TCTACACT2698094.162762599766813Illumina Single End PCR Primer 1 (100% over 8bp)
TCGACGAG1986813.0653604441818847No Hit
GATCGTGT1787372.757653372550659No Hit
TCTTTCCC1531152.3623429739678645Illumina Single End PCR Primer 1 (100% over 8bp)
GACACTGA1510312.3301898684083238No Hit
TACGAGAC1448882.235412264064631TruSeq Adapter, Index 14 (100% over 8bp)
GCTCTAGT1406652.17025748250132No Hit
AGAGTCAC1383422.1344169526477637No Hit
CTAGAGCT1341372.069539885048019No Hit
ACGTCTCG1266321.9537485907944918No Hit
TGCGTACG1184511.8275275943537048No Hit
CGTCGCTA1103041.7018311687329872No Hit
CGTTACTA1074001.6570266492776584No Hit
TAGCGAGT1026881.5843273050374689No Hit
ACTATCTG953281.470773151046002No Hit
GGATATCT788631.2167420171506886No Hit
TCATCGAG780641.2044146028790608No Hit
GTCAGATA759401.1716443551782623No Hit
CTGCGTGT721931.1138335650959217No Hit
GACACCGT580990.8963835316236747No Hit
ATCGTACG535850.8267390409827124RNA PCR Primer, Index 22 (100% over 8bp)
CGTGAGTG380240.5866553194798294No Hit
CTACTATA339050.5231051074837896No Hit
CGCTCTAC82990.128041565757498No Hit
CTAGTATT76500.1180284345155874No Hit
GATCGCGT68640.10590159143986821No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)