FastQCFastQC Report
Thu 20 Oct 2022
000000000-G98TK_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G98TK_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6481489
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTACG2804014.326181838771924No Hit
ACTCACTG2649734.088150114888724No Hit
TAGTCTCC2482193.8296601290228214No Hit
CGAGCGAC2478893.824568706357444No Hit
GTCTGCTA2455763.788882462039201No Hit
GACATAGT2275413.510628499099513No Hit
ACGCTACT2126323.2806041944991344No Hit
GTCTATGA2092883.229011111489968No Hit
ACTACGAC2061133.1800254540276165No Hit
CGAGAGTT1801492.779438490137066TruSeq Adapter, Index 13 (100% over 8bp)
CGAAGTAT1450612.238081403825572No Hit
ATAGTACC1428252.203583158129251No Hit
TAGCAGCT1420722.1919654573200695No Hit
GATCTACG1318582.034378211549846No Hit
AACGCTGA1303412.0109730958426373No Hit
TCTCTATG1122321.7315774199416216ABI Solid3 Adapter B (100% over 8bp)
TAACGTCC1027031.5845587333404405No Hit
CGTCATAC1026421.5836175915750224No Hit
AGCATACC903911.3946023822612366No Hit
TGCTCGTA886571.3678492704377034No Hit
TCTAGACT870951.3437498698215795No Hit
CTGCGTAG844901.303558487872154No Hit
TATAGCGA831121.2822979411058169No Hit
GTAACGAG810661.2507311205804716No Hit
ATAGCGCT770761.1891711919899886No Hit
TCAGTCTA748921.1554752310773033No Hit
CATCGTGA745961.1509083792319943No Hit
CTCGACTT736231.1358963966458941No Hit
TACTAGGT728081.1233221255177628No Hit
CTCTAGAG701361.0820970304817303No Hit
CGAGCTAG697571.076249608693311No Hit
GAGACTTA688901.0628730527815444No Hit
GCGATACG663181.023190813098657No Hit
ACGTGCGC637010.9828142885068539No Hit
CGCGATAT631500.9743131555110254No Hit
GTTACAGC598700.9237074999278715No Hit
GCGTATAC595860.9193257907249399No Hit
ATGAGCTC584370.9015983827173047No Hit
CTACGACC576940.8901349674434378No Hit
ACTGTGTA563360.8691829917477295No Hit
TCCTCATG542520.8370298861881892No Hit
CTATCGTG478790.7387037145322626No Hit
TGCGTCAA398180.6143341445152495No Hit
ACGTACGT393620.6072987241049086No Hit
AGTCGCAG206090.3179670597296393No Hit
CGTAGCGA118990.18358435847071558No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)