FastQCFastQC Report
Thu 8 Oct 2020
000000000-G6GV3_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G6GV3_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467020
Sequences flagged as poor quality0
Sequence length287
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT141453.028778210783264No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT51681.1065907241659887No Hit
TTCTCTCCTCCCTCCTTCTCTTCTTTCCTTTTCCTTCCCTCCCTTCTCCT4680.10020984112029463No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGG109.790009E-4280.999974
CGAAACG109.790009E-4280.999973
CCAATCG109.790009E-4280.999973
CGATGCG109.790009E-4280.999973
GATACGA21450.0279.034974
GCTATCG6150.0278.715453
GCGATAC21550.0278.392122
GGCGATA21500.0277.079071
CTATCGA6400.0276.609344
CCGCTAT6550.0274.564881
TCGACAG6400.0274.414037
TACGACA22150.0272.119636
CGCTATC6750.0266.429632
TATCGAC6950.0256.7415
CGATACA252.396937E-7224.83
CGCTAAC202.769504E-5210.749982
CCTATCG202.769504E-5210.749983
CGATACG29700.0202.471393
CGACAGC40550.0194.032068
ATACGAC31300.0192.121415
Produced by FastQC (version 0.11.5)