FastQCFastQC Report
Thu 8 Oct 2020
000000000-G6GV3_l01_n02_JRS_021_C9T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G6GV3_l01_n02_JRS_021_C9T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1361996
Sequences flagged as poor quality0
Sequence length287
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT57623742.30827403311023No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT45178333.17065542042708No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT54810.4024240893512169No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG54590.40080881294805565No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGCGAGCGCTATGAGCGACT52020.38193944769294474No Hit
CCGCTATCGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG47930.35190999092508346No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC43000.3157131151633338No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC40080.29427399199410276No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA39530.29023580098619967No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG38890.28553681508609424No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG38390.28186573235163687No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCACTA35750.26248241551370194No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG32600.2393545942866205No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA30810.2262121180972631No Hit
CCGCTATCGACCTATGGGCGAGCGCTATGAGCGACTATGGCTATGGGAGA30370.22298156529094065No Hit
GGCGATACGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC28400.2085174993171786No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA28150.20668195794994992No Hit
GGCGATACGACAGCAGTATCTGCTGTCTATGGGCGAGCGCTATGCTATGG27410.20124875550295301No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC26640.1955952880918887No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT26370.19361290341528167No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGTGACTGCACGACAGTCGACT25770.18920760413393284No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG25300.1857567863635429No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG23730.17422958657734677No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC23110.16967744398661963No Hit
GGCGATACGACAGCAGTATCTGCTATGGGCGAGCGCTATGAGCGACTATG22920.16828243254752584No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGACAGCT22210.16306949506459636No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGAGCGAGCGCT21210.15572732959568164No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG20810.15279046340811575No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC20670.15176256024246768No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG19690.14456723808293123No Hit
CCGCTATCGACAGCAGTATCTGCTGTCCTATGGGCGAGCGCTATGAGCGA19560.1436127565719723No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGCTATGGGAGAGCGCGCTCG19030.1397214088734475No Hit
CCGCTATCGACAGCAGTATCTCTATGGGCGAGCGCTATGAGCGACTATGG18140.1331868816061134No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACG18080.13274635167797852No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGCCTGCACGACAGTCGACT17120.12569787282782036No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG17010.12489023462623973No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGGGCG16600.12187994678398467No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG15950.1171075392291901No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGAGAGC15510.11387698642286763No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG15390.11299592656659783No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG15350.11270223994784125No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACAGCACGACATGGGAGA15290.11226171001970638No Hit
GGCGATACGACCGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT15170.1113806501634366No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC14950.10976537376027537No Hit
GGCGATACGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG14690.10785641073835753No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGAG14220.1044055929679676No Hit
GGCGATACGACAGCAGTCTATGGGAGAGCGCGCTCGTCGACTATGGGTCA13740.10088135354288852No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGTCT13630.10007371534130792No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATA732450.0280.290251
CCGCTAT585650.0280.25631
CGATACG735750.0280.140663
GCGATAC734250.0280.08152
GATACGA733950.0279.98544
CTATCGA582850.0279.77064
GCTATCG582600.0279.577183
ATACGAC735350.0279.50975
TCGACAG583100.0277.530247
CGCTATC589650.0277.330542
TACGACA740750.0276.65656
CGACAGC1329700.0276.276868
TATCGAC590450.0276.074345
GCGATCC850.0181.823532
GCCGATA2500.0179.841
CCTATCG2000.0175.6253
GATACGC3200.0162.453144
GCTAACG2650.0159.05663
CGACCGC6200.0158.629038
CGATCCG900.0156.111113
Produced by FastQC (version 0.11.5)