FastQCFastQC Report
Thu 8 Oct 2020
000000000-G6GV3_l01_n02_JRS_021_C9A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G6GV3_l01_n02_JRS_021_C9A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972234
Sequences flagged as poor quality0
Sequence length287
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT68527670.48467755704903No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT112621.1583631101154659No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG92660.9530627400399493No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC91290.9389714821740445No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG87740.90245763879889No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT84300.867075210288881No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC78090.8032016983565685No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG57550.5919356862648293No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG53830.5536732926435406No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA51730.5320735543089421No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA45270.465628644955844No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC39900.41039502835737074No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG39550.406795071968271No Hit
GGCGATACGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG35970.3699726609026222No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA35880.3690469578311394No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG35800.368224110656488No Hit
GGCGATACGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC34080.3505328964014836No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG31480.32379036322531407No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGAG29520.3036306074463555No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC29180.3001335069540872No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC27220.2799737511751286No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACCTATGGGCGAGCGCTA26190.26937959380149223No Hit
GGCGATACGACAGCAGTATCTGCTGTCCTATGGGCGAGCGCTATGAGCGA26120.26865960252367227No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT24960.25672831849122746No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTACTATGGGCGAGCGC23980.24664844060174815No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGACAGC21950.22576869354496962No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGAGAGCGC20350.20931175005194222No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGCTATGGGCGAGCGCTATG19600.20159755778958563No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGAGAGCGCG18350.18874057068565797No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC15930.16384944365245405No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACCTATGGGAGAGCGCGC15630.16076376674751142No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAG15330.15767808984256876No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGAGAGC14870.15294671858832337No Hit
GGCGATACGACAGCAGTATCTGCTGTCTATGGGCGAGCGCTATGAGCGAC14170.1457468058101239No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATA13530.13916402841291295No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGTCAGCAGT13480.13864974892875584No Hit
GGCGATACGACAGCAGTATCTGCTGTCACTATGGGCGACTATGGGACAGC13020.13391837767451045No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGAGAGCGCGCT12330.12682132079314237No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTATGGG11600.11931284032444864No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA11410.11735857828465163No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCTA10900.11211292754624916No Hit
GGCGATACGACAGCAGTATCTGCTGTCATACTATGGGCGAGCGCTATGAG10830.11139293626842922No Hit
GGCGATACGACAGCAGTATCTGCTACTATGGGCGAGCGCTATGAGCGACT10520.10820440346665514No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGCTATGGGCGACTATGGGACA10430.10727870039517234No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGACTATGGGCGAGCGCTATG10340.10635299732368957No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC932850.0280.216835
GATACGA933100.0280.171844
CGATACG935350.0280.15883
GGCGATA937200.0280.145481
GCGATAC937200.0279.980562
TACGACA933750.0279.78126
CGACAGC943300.0276.412488
ACGACAG1690550.0154.208837
GATACGC4700.0140.54
CTCGCAT800.0140.53
ATACGCT4950.0133.404045
TGACAGC2950.0128.593228
CGTTACG1100.0127.727283
TATGACA2650.0127.245296
GACAGCA2066000.0126.007969
TACGCTA5300.0124.5943456
GGCCCGC404.382528E-4105.3751
GCGATAT3400.095.044112
GGCTCGC1053.6925485E-1093.666671
CTCCGAG850.090.91176280-281
Produced by FastQC (version 0.11.5)