FastQCFastQC Report
Thu 8 Oct 2020
000000000-G6GV3_l01_n02_JRS_021_C8T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G6GV3_l01_n02_JRS_021_C8T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970929
Sequences flagged as poor quality0
Sequence length287
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT25245026.00087133044744No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT23220623.915857905160934No Hit
GTCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT74430.7665854042880582No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGAGTATGGGACTACA41400.4263957508736478No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCACTA29070.2994039728960614No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG27540.28364586905942657No Hit
TGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT27290.281071015491349No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG26370.2715955543608235No Hit
CCGCTATCGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG26150.2693296832209152No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCCC26020.2679907593655149No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC25050.25800032752137386No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC23780.24492007139553973No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACAGTACAGTATAGTTAC22830.2351356278368449No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACCCACAGCTTTAAGGCC22820.23503263369412183No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA22640.23317873912510595No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC20650.2126829047232084No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG20360.20969607458423842No Hit
GGCGATACGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC19890.20485534987625253No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG19230.19805773645652772No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC18710.19270204103492636No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTACAGTCGCTACGACT18070.18611041590064775No Hit
GGCGATACGACAGCAGTATCTGCTGACATGGGACTGCACGACAGTCGACT17980.1851834686161398No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA17910.18446250961707808No Hit
CCGCTAACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT16780.1728241714893674No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG16510.17004332963584362No Hit
CCGCTATCGACAGCAGTATCTGCTGACATGGGACTGCACGACAGTCGACT16260.16746847606776602No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCAGT16030.16509961078513463No Hit
GGCGATACGACAGCAGTACTATGGGACAGAGATATCATGCAGTATGGATC15940.16417266350062673No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGAGTATGGATCGACA15720.16190679236071845No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGCGAGCGCTATGAGCGACT15530.15994990364897949No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG14780.15222534294474674No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGAACTGCACGACAGTCGACT14570.15006246594756156No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG14570.15006246594756156No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGTCT14540.14975348351939224No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCGAG14480.14913551866305363No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG14040.1446037763832371No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT13840.14254389352877503No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGAACTGCACGACAGTCGACT13770.14182293452971328No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG13710.14120496967337468No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA13320.13718819810717364No Hit
GGCGATACGACAGCAGTATCTACTGTCATGGGACTGCACGACAGTCGACT13220.1361582566799426No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACAGCACGACAGTCGACT12910.13296543825552642No Hit
CCGCTATCGACTATGGGAGAGCGCGCTCGTCGACTATGGGTCAGCAGTAC12790.13172950854284918No Hit
CCCCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT12740.13121453782923365No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGACAGCT12550.12925764911749468No Hit
CCGCTATCGACAGCAGTATCTGCTGTCCTATGGGCGAGCGCTATGAGCGA12130.12493189512312435No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC11890.12246003569776986No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACAGCACGACAGTCGACT11580.11926721727335365No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGAGCGAGCGCT11400.1174133227043378No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACG11170.11504445742170644No Hit
CCGCTATCGACAGCAGTATCTACTGTCATGGGACTGCACGACAGTCGACT10930.11257259799635196No Hit
GGCGATACGACAGCAGTATCTGCTATGGGCGAGCGCTATGAGCGACTATG10840.11164565071184401No Hit
GGCGATACGACAGCAGTATCTGCTATCATGGGACTGCACGACAGTCGACT10610.10927678542921263No Hit
CCTCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT10290.10598097286207334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCCC400.0280.999972
GGCGATA431600.0280.707031
GCGATAC436950.0280.5822
CCGCTAT426400.0280.439851
GCTATCG420700.0280.03153
CTATCGA425550.0279.646334
CGCTATC426400.0278.660522
TCGACAG421850.0277.50297
GCTAACG6100.0276.393463
CGACAGC896050.0276.045148
CGATACG458850.0276.039553
ATACGAC468650.0275.723575
TATCGAC432900.0275.547455
GATACGA461350.0275.09194
TACGACA472350.0272.939156
TGATACG2400.0263.43753
CGCTAAC6350.0263.299222
CCGCTAA6500.0257.223081
CAATACG11350.0247.577093
GTGATAC2350.0245.127672
Produced by FastQC (version 0.11.5)