FastQCFastQC Report
Thu 8 Oct 2020
000000000-G6GV3_l01_n02_JRS_021_C8A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G6GV3_l01_n02_JRS_021_C8A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194690
Sequences flagged as poor quality0
Sequence length287
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT60935551.00528170487741No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT175671.470423289723694No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT107100.8964668658815258No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG92400.7734223940938654No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG88370.7396897940051393No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC85570.7162527517598708No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC79850.6683742226016791No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG73240.6130460621583842No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA72040.6030016154818405No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGT68840.5762164243443906No Hit
GGCGATACGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC60800.5089186316115477No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG57060.4776134394696532No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA46920.3927378650528589No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC45790.38327934443244693No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACAGCACGACAGTCGACT45700.38252601093170613No Hit
GGCGATACGACAGCAGTATCTGCTGACATGGGACTGCACGACAGTCGACT44000.3682963781399359No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG39630.33171785149285593No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA36240.3033422896316199No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACCTATGGGCGAGCGCTA36210.30309117846470635No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAG35580.2978178439595209No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA34860.29179117595359466No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC34140.2857645079476684No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG31690.2652570959830584No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTACTATGGGCGAGCGC28450.23813708995639038No Hit
GGCGATACGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG27580.2308548661158962No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC26460.22148004921778872No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGCTATGGGCGAGCGCTATG26180.21913634499326184No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCATATAGTATGGGA25520.2136118993211628No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG24170.20231189681005116No Hit
GGCGATACGACAGCAGTATCTGCTGTCCTATGGGCGAGCGCTATGAGCGA23770.19896374791786992No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGAGAGC22690.18992374590898056No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGAG21410.17920966945400063No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGA20020.16757485205367081No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19590.163975591994576No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATA17710.1482392922013242No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACCTATGGGAGAGCGCGC16010.13400965940955395No Hit
GGCGATACGACAGCAGTATCTACTGTCATGGGACTGCACGACAGTCGACT15760.13191706635194067No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAT15400.12890373234897756No Hit
GGCGATACGACAGCAGTATCTGCTGTCATAGGACTGCACGACAGTCGACT15150.12681113929136428No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGAC14590.12212373084231057No Hit
GGCGATACGACAGCAGTATCTAGTATGGATCGACAAATGGCAGTATTCAT14290.11961261917317464No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGAGTATGGGACTACAGTCG14090.11793854472708402No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGAGAGCGCG13940.11668298889251606No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC13700.11467409955720732No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATC13470.1127489139442031No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGGTATGG13130.10990298738584904No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT13080.1094844687743264No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGACTATGGGCGAGCGCTATG13010.10889854271819467No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGAGAGCGC12900.10797780177284484No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGAACTGCACGACAGTCGACT12600.1054666901037089No Hit
GGCGATACGACAGCAGTATCTATGGGCGAGCTATGGAGTATGGGACCTAT12590.10538298638140438No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGTAT12140.10161631887770049No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTTATGGGCGAGCGCTA12070.10103039282156878No Hit
GGCGATACGACAGCAGTATCTGCTGACATGGGACAGCACGACAGTCGACT12050.10086298537695972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAC1079350.0280.830782
GGCGATA1079550.0280.66161
ATACGAC1073050.0279.690645
GATACGA1075250.0279.601874
CGATACG1080750.0279.465973
TACGACA1071650.0279.374276
CGACAGC1078750.0277.066658
GATACGT850.0247.941184
GGCGATC1600.0219.531251
ATACGCT5800.0203.482765
ATACGTC850.0198.352955
GATACCT1000.0196.74
TACGCTA6200.0190.354836
GTGATAC700.0180.642852
GCGAAAC6150.0178.195112
CAATACG1900.0177.473683
TATGACA2600.0172.923086
CGAAAGC3750.0168.68
TACGTCG256.744893E-5168.66
TGACAGC3350.0167.76128
Produced by FastQC (version 0.11.5)