FastQCFastQC Report
Thu 8 Oct 2020
000000000-G6GV3_l01_n01_JRS_021_C8T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G6GV3_l01_n01_JRS_021_C8T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970929
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTG3750.021.4133324
GGGCTAC3500.021.0571441
AAGGGAG1700.020.7058837
GGGCGTG2200.020.52
CGACTCA3550.020.4507054
AACGACC1750.020.1142862
ATGAGGT4050.020.0987643
ACGGAAG6250.020.0649
GGGAGGG5100.020.0588259
TAACGAC2250.020.0444451
TGGATGG551.264998E-720.02
TGCCGGA551.264998E-720.011
CAATTAG9300.019.98924610
GCACGAC6450.019.9534877
GTCGCAC5900.019.9491524
GCACTCC3650.019.89041110
GAACACT7750.019.7290331
GTGCGTG2400.019.7083321
TAGAGCC5150.019.6504847
AATAGGC3700.019.6216225
Produced by FastQC (version 0.11.5)