FastQCFastQC Report
Thu 8 Oct 2020
000000000-G6GV3_l01_n01_JRS_021_C8A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G6GV3_l01_n01_JRS_021_C8A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194690
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA801.8189894E-1220.6250024
GGGCGGT451.0019432E-519.5555553
GACCCGA3850.019.4285722
ACCCGAG3850.019.4285723
GAAGCCG2050.019.3170746
GTGCGTG4400.019.251
GCCAACG4000.019.251
AGGCAGA3950.019.2151895
CGCTGAG3550.019.2112673
CCCGGAA3100.019.1612911
AAAGGAG2600.019.0384626
AGAAAAG4050.019.0123463
TACACCC5150.019.009711
AGCAACG3500.018.8571436
AAAAGTG4000.018.75
CAGGCAG4550.018.6153854
AGACAGG4650.018.4516141
ACCCTCG4600.018.4130424
GGACGGA2450.018.4081639
GCCACTC4900.018.40816310