FastQCFastQC Report
Fri 28 Aug 2020
000000000-G69NY_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NY_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796480
Sequences flagged as poor quality0
Sequence length287
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT26625714.821038920555754No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT1529168.51197898111863No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT590913.2892656750979694No Hit
TGCAACACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT402282.2392679016743857No Hit
ACTACTTAGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT192901.073766476665479No Hit
TGTGCGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT36680.2041770573566085No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG33860.18847969362308514No Hit
TAAGCGAGGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT28550.15892189169932314No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG28540.15886622728892055No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC26050.14500578909868186No Hit
GTACTGACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT25580.1423895618097613No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG24810.1381034022087638No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC24170.13454087994299962No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA18500.10297915924474528No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT18100.10075258282864269No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGACA366850.0280.425546
TGATGAC362850.0280.380465
TGACAGC370150.0280.01318
GCGATAC166050.0279.81542
GGCGATA165150.0279.46871
ATGACAG372150.0278.130747
GATACGA168750.0277.586364
TACGACA171650.0275.434026
CTATCGA63650.0275.040074
GCTATCG62100.0274.891333
CCGCTAT61500.0274.603271
GCAACAC43650.0271.665532
CAACACG43650.0271.665533
TCGACAG66500.0270.43617
TATCGAC64950.0270.1845
GTGCGAT4350.0268.080442
CGCTATC64000.0266.29142
TACTTAG20300.0263.697023
GTACTGA386750.0263.271761
TACTGAT390550.0259.558962
Produced by FastQC (version 0.11.5)