FastQCFastQC Report
Fri 28 Aug 2020
000000000-G69NY_l01_n02_WGS17-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NY_l01_n02_WGS17-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975
Sequences flagged as poor quality0
Sequence length287
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT1939.772151898734178No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT1748.810126582278482No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT361.8227848101265824No Hit
TGCAACACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT331.6708860759493671No Hit
ACTACTTAGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT180.9113924050632912No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG50.25316455696202533No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG50.25316455696202533No Hit
GCTAAAGGTGCTTTGACTTATACCGATATTGCTGGCGACCCTGTTTTGTA30.1518987341772152No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG30.1518987341772152No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG20.10126582278481014No Hit
GTACTGATGACAGCAGTATCTGCTGTCACTATGGGAGAGCGCGCTCGTCG20.10126582278481014No Hit
TTACCGCTTCGGCGTTATAACCTCACACTCAATCTTTTATCACGAAGTCA20.10126582278481014No Hit
TCTCAAAAACATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTCTC20.10126582278481014No Hit
CATTGGGGATTGAGAAAGAGTAGAAATGCCACAAGCCTCAATAGCAGGTT20.10126582278481014No Hit
ACAAAATGTGACTCATATCTAAACCAGTCCTTGACGAACGTGCCAAGCAT20.10126582278481014No Hit
AAAAAATTTAGGGTCGGCATCAAAAGCAATATCAGCACCAACAGAAACAA20.10126582278481014No Hit
ATCTCTCTTTTTGCGTTCTGCTTCAATATCTGGTTGAACGGCGTCGCGTC20.10126582278481014No Hit
GTTCAGAATCAGAATGAGCCGCAACTTCGGGATGAAAATGCTCACAATGA20.10126582278481014No Hit
AAATCATGAGTCAAGTTACTGAACAATCCGTACGTTTCCAGACCGCTTTG20.10126582278481014No Hit
TGTGCGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT20.10126582278481014No Hit
CCAACAGCCATATAACTGGTAGCTTTAAGCGGCTCACCTTTAGCATCAAC20.10126582278481014No Hit
AGATATTCAAATAACCCTGAAACAAATGCTTAGGGATTTTATTGGTATCA20.10126582278481014No Hit
TGCCTCCAAATCTTGGAGGCTTTTTTATGGTTCGTTCTTATTACCCTTCT20.10126582278481014No Hit
GTTTGAGCTTGAGTAAGCATTTGGCGCATAATCTCGGAAACCTGCTGTTG20.10126582278481014No Hit
GCGCGTACACGCAAGGTAAACGCGAACAATTCAGCGGCTTTAACCGGACG20.10126582278481014No Hit
AAGGTTAGTGCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAATCAGCG20.10126582278481014No Hit
GTACTGATGACAGCAGTATCTGCTGTCCTATGGGCGAGCGCTATGAGCGA20.10126582278481014No Hit
CAACTACTCCGGTTATCGCTGGCGACTCCTTCGAGATGGACGCCGTTGGC20.10126582278481014No Hit
GATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATGAC20.10126582278481014No Hit
TAATATGCTTGGCACGTTCGTCAAGGACTGGTTTAGATATGAGTCACATT20.10126582278481014No Hit
TGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAA20.10126582278481014No Hit
AGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTT20.10126582278481014No Hit
GTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTG20.10126582278481014No Hit
AAATGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGACTTCACGCC20.10126582278481014No Hit
CCATGTCTAAATTGTTTGGAGGCGGTCAAAAAGCCGCCTCCGGTGGCATT20.10126582278481014No Hit
GACAGCAGTATCATCGATACTTCATCGATGTCTATGCTCTCGACTCCATA20.10126582278481014No Hit
GTGTGAATCATTAGCCTTGCGACCCTCGGCAGCAAGAACCATACGACCAA20.10126582278481014No Hit
TCTAATTTGTCTAGGAAATAACCGTCAGGATTGACACCCTCCCAATTGTA20.10126582278481014No Hit
TCTATTATGGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCCGAGAT20.10126582278481014No Hit
CACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGCATA20.10126582278481014No Hit
GGATTAAGCACTCCGTGGACAGATTTGTCATTGTGAGCATTTTCATCCCG20.10126582278481014No Hit
GCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGA20.10126582278481014No Hit
CATACCAAAGACGAGCGCCTTTACGCTTGCCTTTAGTACCTCGCAACGGC20.10126582278481014No Hit
AAGCTCAGGAAATGCAGCAGCAAGATAATCACGAGTATCCTTTCCTTTAT20.10126582278481014No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA20.10126582278481014No Hit
GAGCGCCTTTACGCTTGCCTTTAGTACCTCGCAACGGCTGCGGACGACCA20.10126582278481014No Hit
CTAAAAACCAAGCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAGAAT20.10126582278481014No Hit
GGTCGCAAGGCTAATGATTCACACGCCGACTGCTATCAGTATTTTTGTGT20.10126582278481014No Hit
GATGATGCTCGTTATGGTTTCCGTTGCTGCCATCTCAAAAACATTTGGAC20.10126582278481014No Hit
AGACTTTTATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGTT20.10126582278481014No Hit
CAGCAGTCCACTTCGATTTAATTCGTAAACAAGCAGTAGTAATTCCTGCT20.10126582278481014No Hit
GTCAGTAAGAACGTCAGTGTTTCCTGCGCGTACACGCAAGGTAAACGCGA20.10126582278481014No Hit
ATGTTTTCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTGCTGAGGGT20.10126582278481014No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC20.10126582278481014No Hit
AGATGCAAAATGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGACT20.10126582278481014No Hit
CATACGAAAAGACAGAATCTCTTCCAAGAGCTTGATGCGGTTATCCATCT20.10126582278481014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGAC254.765752E-10281.05
CGATACG157.5098924E-6281.03
GATACGA157.5098924E-6281.04
GCGATAC157.5098924E-6281.02
ATGACAG254.765752E-10281.07
GTACTGA254.765752E-10281.01
TGACAGC254.765752E-10281.08
GGCGATA157.5098924E-6281.01
GATGACA254.765752E-10281.06
ATACGAC157.5098924E-6281.05
TACGACA157.5098924E-6281.06
TACTGAT254.765752E-10281.02
ACTGATG301.4169927E-9234.166663
CTGATGA301.4169927E-9234.166664
CGACAGC304.5690285E-7187.333338
GACAGCA1550.090.6451649
CGGAAGA553.7107384E-1089.409096280-281
ACGACAG651.2823875E-986.461547
GGAAGAG651.3988029E-975.65385280-281
AGTATCA451.325916E-762.4444437
Produced by FastQC (version 0.11.5)