FastQCFastQC Report
Fri 28 Aug 2020
000000000-G69NY_l01_n02_WGS17-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NY_l01_n02_WGS17-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605867
Sequences flagged as poor quality0
Sequence length287
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT35127457.97873130571561No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT6537910.790982179257163No Hit
TGCAACACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT430647.107830596484047No Hit
ACTACTTAGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT254544.2012520899801435No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT209733.4616508243558406No Hit
TGTGCGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT67821.1193875883651032No Hit
CCTCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT35170.5804904376703138No Hit
TAAGCGAGGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT29360.4845948038100771No Hit
GTTTGCATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT25320.4179135024683635No Hit
TGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT11840.19542242769452703No Hit
CCTACTTAGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT11550.19063589863781985No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC10970.18106284052440552No Hit
GGCGATACGACAGCAGATACTGCTGTCATGGGACTGCACGACAGTCGACT9220.15217861345806918No Hit
GTCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT8770.1447512407838684No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG8070.13319754995733388No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG7770.12824596817453335No Hit
GGCGATATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT7710.12725565181797327No Hit
TGCCACACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT6540.10794448286505125No Hit
ACTACTTCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT6410.1057987974258377No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA6360.10497353379537093No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG6220.10266279563006403No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGAC109.790725E-4281.07
GTGACTG109.790725E-4281.09
GGCGATA381250.0280.92631
GCGATAC381850.0280.852842
GCTATCG68900.0280.592163
CCGCTAT68650.0280.59071
CGATACG383150.0280.156623
GATACGA384100.0280.08554
ATACGAC385700.0279.79795
TACGACA386650.0279.36486
CGACAGC507350.0279.033788
GCAACAC44100.0278.769872
CGCTATC69450.0278.167762
CTATCGA73900.0278.148164
TGATGAC23250.0276.16565
TATCGAC74700.0275.92175
CGAGGAC2700.0275.79635
GCGAGGA2650.0275.698124
GATGACA30900.0274.63436
TGCAACA44850.0274.421421
Produced by FastQC (version 0.11.5)