FastQCFastQC Report
Fri 28 Aug 2020
000000000-G69NY_l01_n02_WGS17-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NY_l01_n02_WGS17-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125252
Sequences flagged as poor quality0
Sequence length287
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT5105040.757832210264105No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT2467419.699485836553507No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT78756.2873247532973515No Hit
TGCAACACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT67535.391530674160892No Hit
ACTACTTAGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT28962.312138728323699No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG8580.6850190016925878No Hit
TAAGCGAGGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT6450.5149618369367355No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG5630.4494938204579567No Hit
GTTTGCATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT5560.44390508734391465No Hit
TGTGCGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT4560.3640660428575991No Hit
GTACTGATGACAGCAGATACTGCTGTCATGGGACTGCACGACAGTCGACT4350.3472998435154728No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC3970.3169610066106729No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC3710.29620285504423083No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACTGCGCTAT3600.28742056015073614No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA3460.2762430939226519No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT3280.26187206591511514No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT3230.25788011369079933No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG3020.24111391434867308No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC2930.23392840034490467No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG2710.21636381055791526No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC2670.2131702487784626No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC1950.1556861367483154No Hit
GTACTGATGACAGCAGTATCTATGGGCGAGCGCTATGAGCGACTATGGGA1930.15408935585858907No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG1800.14371028007536807No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA1800.14371028007536807No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGAGAG1770.1413151087407786No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT1730.13812154696132595No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGAG1600.12774247117810494No Hit
TTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT1450.11576661450515761No Hit
GTACTGATGACAGCAGTATCTGCTATGGGAGAGCGCGCTCGTCGACTATG1300.10379075783221027No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGCTATGGGAGAGCGCGCTCGT1290.1029923673873471No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGCTATGGGTCAGCAGTACTA1280.10219397694248396No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG1270.1013955864976208No Hit
GTACTGATGACAGCAGTATCTGCTGCTATGGGTCAGCAGTACTACTGACG1260.10059719605275763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCA307.2759576E-12281.000031
GAGGACA550.0281.06
CGAGGAC550.0281.05
GCGATAC24900.0281.02
GCGAGGA550.0281.04
GATACGA25000.0280.438024
TGACAGC67300.0280.37378
GATGACA67050.0280.371376
TGATGAC66600.0280.367135
GTACTGA66500.0280.366151
GGCGATA25000.0279.8761
CGATACG25050.0279.317353
CCGCTAT7850.0279.21021
TACTGAT66900.0279.109862
GCAACAC6350.0278.787382
TACGACA25250.0278.774266
ACTGATG67000.0278.693273
ATGACAG67800.0278.306067
TCGACAG8100.0277.530857
GCTATCG7850.0277.420383
Produced by FastQC (version 0.11.5)