FastQCFastQC Report
Fri 28 Aug 2020
000000000-G69NY_l01_n02_WGS17-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NY_l01_n02_WGS17-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646042
Sequences flagged as poor quality0
Sequence length287
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT48991375.83299537800949No Hit
GTCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT47970.7425213840586216No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG27210.42118004711767965No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG26910.4165363861792268No Hit
TGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT25780.39904526331105405No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT25300.3916154058095294No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG24410.3778392116921191No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA24220.3748982264310989No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC20340.31484021162710785No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT19990.30942260719891274No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT19350.2995161305302132No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC17170.26577219437745536No Hit
CCAGAGCGTCGCTGTCCGTGTGCTCCCGCAGGATTCGCAGGTGCGTGGAG16380.2535438872395293No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG15570.24100600270570643No Hit
TGCAACACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT14870.2301707938493163No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC14030.21716854322164814No Hit
CCAGAGCGTCGCTGTCCGTGTGCTCCCGCAGGATTCGCAGGTGCGTGGCG13350.20664291176115485No Hit
GGCGTTACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT11330.17537559477557185No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC10740.16624306159661445No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG10720.1659334842007176No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA10680.16531432940892388No Hit
GGCGATATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT10620.1643855972212333No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC10290.1592775701889351No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGAG9650.14937109352023553No Hit
ACTACTTAGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT9030.13977419424743281No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG8450.13079644976642385No Hit
GGCGATACGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC8330.1289389853910427No Hit
GGTGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT7980.12352138096284762No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACCTATGGGCGAGCGCTA7860.12166391658746645No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT7790.12058039570182744No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG6810.10541110330288123No Hit
GGCGATACGACAGCAGTATCTGCTGTCTATGGGCGAGCGCTATGAGCGAC6530.10107701976032517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATA584000.0280.830541
GCGATAC586250.0280.639432
CGATACG592300.0280.239843
GATACGA593250.0280.19374
ATACGAC594450.0279.888065
TACGACA596300.0279.443856
CGACAGC604750.0277.467568
CCAGAGC4400.0249.067211
GCTATCG2950.0238.134673
GGCGATC950.0236.630661
GCAACAC1800.0234.165772
GTCGATA7500.0226.672451
GGCAACC252.397701E-7224.799136
TCGCTGT5250.0216.77069
TGCGATA4050.0215.08561
CTATCGA3650.0211.71154
TGATGAC1400.0210.74925
CCGCTAT3400.0210.749181
TGACAGC4950.0210.03968
GGCGTTA2750.0199.253781
Produced by FastQC (version 0.11.5)