FastQCFastQC Report
Fri 28 Aug 2020
000000000-G69NY_l01_n02_WGS17-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NY_l01_n02_WGS17-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557804
Sequences flagged as poor quality0
Sequence length287
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT36017064.56927522929202No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT218223.9121268402521316No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT98841.771948569748514No Hit
TGCAACACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT67021.2014973001269262No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG48730.8736043484808285No Hit
ACTACTTAGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT34890.6254885228503202No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC29740.5331621860008174No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG27380.4908534180464823No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG25380.45499852994958806No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA25320.4539228833066812No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT24520.4395809280679235No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG23030.4128690364357373No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC22280.39942345339940194No Hit
GTACTGATGACAGCAGATACTGCTGTCATGGGACTGCACGACAGTCGACT21200.38006181382707904No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC20840.37360793396963804No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCAGT19420.3481509634208432No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT16210.2906038680253279No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG14830.2658639952384708No Hit
CCAGAGCGTCGCTGTCCGTGTGCTCCCGCAGGATTCGCAGGTGCGTGGCG14500.25994793870248334No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG14310.2565417243332784No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA13450.24112412245161385No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC13190.23646298699901758No Hit
GTACTGATGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC12600.22588579501043377No Hit
CCAGAGCGTCGCTGTCCGTGTGCTCCCGCAGGATTCGCAGGTGCGTGGAG12580.22552724612946484No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC11720.21010964424780032No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG11430.20491068547375063No Hit
TTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT10180.18250138041319172No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC9650.17299983506751476No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGAG9270.16618740632910484No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA8580.15381746993567633No Hit
GTACTGATGACAGCAGTATCTGCTGTCCTATGGGCGAGCGCTATGAGCGA7780.13947551469691863No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACAGACTATGGGC7730.1385791424944963No Hit
GTACTGATGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG6390.11455636746957713No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACCTATGGGCGAGCGCTA6290.11276362306473242No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGGGCGAGCGCT6110.10953668313601193No Hit
TGTGCGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT5990.10738538985019827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGAC470950.0280.850835
GATGACA471900.0280.79166
GTACTGA469550.0280.640931
TGACAGC473650.0280.52548
CTGATGA472500.0279.72144
TACTGAT472150.0279.601382
ATGACAG474500.0279.489877
ACTGATG473000.0279.336583
GGCGATA22600.0279.134951
GCGATAC22550.0279.13082
GATACGA23150.0271.289434
GCTATCG10500.0270.295233
CGATACG23250.0270.12263
CCAGAGC3250.0268.03081
ATACGAC23750.0264.43585
CCGCTAT11100.0259.4821
TATCGAC11200.0258.419655
CTATCGA11150.0258.31844
TTGCATG600.0257.583343
GCAACAC7200.0255.631942
Produced by FastQC (version 0.11.5)