FastQCFastQC Report
Fri 28 Aug 2020
000000000-G69NY_l01_n02_WGS17-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NY_l01_n02_WGS17-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501118
Sequences flagged as poor quality0
Sequence length287
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT19671139.25442710100216No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT296295.912579472299937No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT137222.7382772121536245No Hit
TGCAACACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT73331.4633279985951413No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC63011.2573884793601509No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG60891.2150830742459862No Hit
ACTACTTAGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT49840.9945761277782877No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG37140.7411428046887161No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG36470.7277727002422584No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG34670.69185301665476No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA33770.6738931748610109No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC31920.6369757222849708No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCAGT30680.6122310513691386No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG21240.4238522663324805No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT16630.3318579655889431No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT15660.3125012472112357No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG14910.29753471238311135No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC14780.2949405130129032No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACTGCTATGG14530.28995166807019507No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC14510.2895525604747784No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA14070.28077219337561216No Hit
GTACTGATGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC13950.27837754780311225No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC13940.27817799400540394No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA13560.2705949496924876No Hit
CCAGAGCGTCGCTGTCCGTGTGCTCCCGCAGGATTCGCAGGTGCGTGGCG13520.2697967345016543No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCAG12860.2566261838529049No Hit
GTACTGATGACAGCAGATACTGCTGTCATGGGACTGCACGACAGTCGACT12510.24964180093311356No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCTCGCTATGGGCGA12430.248045370551447No Hit
CCAGAGCGTCGCTGTCCGTGTGCTCCCGCAGGATTCGCAGGTGCGTGGAG12100.24146009522707226No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTATGGG11320.22589489900582296No Hit
GTACTGATGACAGCAGTATCTATGGGCGAGCGCTATGAGCAGTATCATCG10060.20075112049457414No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACATGGGCGA9620.19197075339540787No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACCTATGGGCGAGCGCTA9380.1871814622504081No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATA9030.18019707933061674No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGT8780.17520823438790864No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG8680.1732126964108254No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGGGCG8470.1690220666589506No Hit
GTACTGATGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG8400.16762519007499233No Hit
TGTGCGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT8070.1610399147506176No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAT8040.16044125335749262No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT7970.1590443767735344No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACG7590.15146133246061808No Hit
GTACTGATGACAGCAGTATCTGCTATGGGCGAGCGCTATGAGCGACTATG7510.14986490207895145No Hit
TAAGCGAGGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT7350.14667204131561828No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA7290.14547471852936833No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCCAT7010.13988721219353525No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC6940.138490335609577No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAACTATGG6630.13230416788061894No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGGGC6630.13230416788061894No Hit
GTACTGATGACAGCAGTATCTGCTGTCCTATGGGCGAGCGCTATGAGCGA6430.12831309192645246No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCATG6170.12312469318603603No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG5990.11953272482728619No Hit
GTACTGATGACAGCAGTATCTGCTGTCTATGGGCGAGCGCTATGAGCGAC5830.11633986406395301No Hit
GTACTGATGACAGCAGTATCTGCTGTCTATGGGAGAGCGCGCTCGTCGAC5790.1155416488731197No Hit
TTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT5720.11414477228916142No Hit
GTACTGACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT5630.1123487881097865No Hit
GTACTGATGACAGCAGTATCTGCTGTCATCTATGGGAGAGCGCGCTCGTC5510.10995414253728664No Hit
GTTTGCATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT5120.10217154442666199No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGCTATGGGCGAGCGCTATG5110.10197199062895365No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGCG158.788227E-6281.03
GATGACA348500.0280.395266
TGATGAC348650.0280.355265
TGACAGC350600.0279.437138
ATGACAG350200.0279.39527
GTACTGA350550.0279.356751
GGCGATA28150.0279.003571
TACTGAT354000.0276.753272
GCGATAC28600.0276.578642
CTGATGA353500.0276.42934
ACTGATG353950.0276.276343
GATACGA29150.0272.806184
GCTATCG15500.0271.029053
TATCGAC15800.0268.550665
CCAGAGC2950.0266.711881
CCGCTAT15950.0266.02511
CTGGGCT850.0264.470584
CGATACG30150.0263.291843
CAACACG7800.0261.18593
TACGACA30900.0259.629466
Produced by FastQC (version 0.11.5)