FastQCFastQC Report
Fri 28 Aug 2020
000000000-G69NY_l01_n02_WGS17-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NY_l01_n02_WGS17-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687632
Sequences flagged as poor quality0
Sequence length287
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT49882072.54170835563208No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG38180.5552388486865067No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG36790.535024548014054No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG35320.5136468343532586No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC26520.3856714056355725No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA26470.3849442725178584No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC24360.3542592549503223No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG23330.33928031272541126No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT21030.3058321893105615No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT17360.2524606184703446No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG15100.2195942015496661No Hit
TGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT15000.21813993531423784No Hit
GGCGATACGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC14910.21683109570235243No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC14900.2166856690788096No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA14800.21523140284338133No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC14290.20781464504269725No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGAG13440.1954533820415571No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG13080.1902180235940154No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC12540.18236498592270284No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG12060.17538450799264724No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGAATGGGCGAG11820.17189426902761942No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT11430.16622263070944923No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC10460.15211624822579517No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA10250.14906228913139585No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACCTATGGGCGAGCGCTA9470.1377190124950555No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA9090.1321928008004281No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT8340.12128580403471625No Hit
TGCAACACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT8180.11895897805803105No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGT8010.116486725457803No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGAGAGCGCG7800.11343276636340369No Hit
GGCGATACGACAGCAGATACTGCTGTCATGGGACTGCACGACAGTCGACT7750.11270563324568955No Hit
GGCGATACGACAGCAGTATCTATGGGAGAGCGCGCTCGTCGACTATGGGT7750.11270563324568955No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGAGAGCGC7070.10281662284477744No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAC624000.0280.752322
GGCGATA622750.0280.70671
GATACGA622850.0280.639074
CGATACG623700.0280.48193
ATACGAC624250.0280.144745
TACGACA623200.0280.075656
CGACAGC632550.0276.42448
CGAGGTG302.1154847E-9234.166665
CGCGAGG252.3977555E-7224.85
GGTGACT501.8189894E-12196.78
TGGGCTG150.0032956647187.333339
TGATGAC900.0187.333335
GAATGGT401.1812517E-8175.6254
TGCGATA4400.0162.852261
TCGAGGT351.2819528E-6160.571434
GATACCT800.0158.06254
GCTATCG900.0156.111113
CCAGAGC1350.0156.111111
GCGATCT1000.0154.552
ACGACAG1171300.0149.136577
Produced by FastQC (version 0.11.5)