FastQCFastQC Report
Fri 28 Aug 2020
000000000-G69NY_l01_n01_WGS17-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NY_l01_n01_WGS17-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975
Sequences flagged as poor quality0
Sequence length28
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGACTATTTTCGTGATATTGGTCGTA30.1518987341772152No Hit
CAAAGCATTGGGATTATCATAAAACGCC30.1518987341772152No Hit
TGGGAAGCCTTCAAGAAGGTGATAAGCA20.10126582278481014No Hit
TCCAAGCAACAGCAGGTTTCCGAGATTA20.10126582278481014No Hit
AAGGTAAAAAACGTTCTGGCGCTCGCCC20.10126582278481014No Hit
AGGCTTGTGGCATTTCTACTCTTTCTCA20.10126582278481014No Hit
CAATGCGACAGGCTCATGCTGATGGTTG20.10126582278481014No Hit
ATCTTGATAAAGCAGGAATTACTACTGC20.10126582278481014No Hit
ATATACCTGGTCTTTCGTATTCTGGCGT20.10126582278481014No Hit
CTTAATCCACTGTTCACCATAAACGTGA20.10126582278481014No Hit
AAACATAAATCACCTCACTTAAGTGGCT20.10126582278481014No Hit
ATGTAGCTTTAGGTGTCTGTAAAACAGG20.10126582278481014No Hit
AGCCGACGTTTTGGCGGCGCAACCTGTG20.10126582278481014No Hit
GGGATCCTCTTGTGGATGAATACTGCCA20.10126582278481014No Hit
ATAATTCAAACTTTTTTTCTGATAAGCT20.10126582278481014No Hit
CGGGGAAGGACGTCAATAGTCACACAGT20.10126582278481014No Hit
TTCAAGAAGTCCTTTACCAGCTTTAGCC20.10126582278481014No Hit
TCTGCGTCATGGAAGCGATAAAACTCTG20.10126582278481014No Hit
GAACAATTCAGCGGCTTTAACCGGACGC20.10126582278481014No Hit
AGAGTAAACATAGTGCCATGCTCAGGAA20.10126582278481014No Hit
CAGATATGGACCTTGCTGCTAAAGGTCT20.10126582278481014No Hit
ATGGAAGGCGCTGAATTTACGGAAAACA20.10126582278481014No Hit
CCATAAACGTGACGATGAGGGACATAAA20.10126582278481014No Hit
TTACTAAAATGCAACTGGACAATCAGAA20.10126582278481014No Hit
AAGCCTCAATAGCAGGTTTAAGAGCCTC20.10126582278481014No Hit
CAAAGCACCTTTAGCGTTAAGGTACTGA20.10126582278481014No Hit
GACTGGTTTAGATATGAGTCACATTTTG20.10126582278481014No Hit
ACGAAAAGACAGAATCTCTTCCAAGAGC20.10126582278481014No Hit
GTCTGAAACATGATTAAACTCCTAAGCA20.10126582278481014No Hit
AAGACCAGGTATATGCACAAAATGAGAT20.10126582278481014No Hit
AAAACATCCTTCATAGAAATTTCACGCG20.10126582278481014No Hit
GATAAACGGTCACATTAAATTTAACCTG20.10126582278481014No Hit
TATAGACCACCGCCCCGAAGGGGACGAA20.10126582278481014No Hit
AGTAGGCGGAAAACGAACAAGCGCAAGA20.10126582278481014No Hit
AATATCTATAACAACTATTTTAAAGCGC20.10126582278481014No Hit
TCAAATCCGGCGTCAACCATACCAGCAG20.10126582278481014No Hit
CGAATATCCTTAAGAGGGCGTTCAGCAG20.10126582278481014No Hit
ACGGTCACATTAAATTTAACCTGACTAT20.10126582278481014No Hit
TTTTTTCTTTTGTGGATGAATACTGCCA20.10126582278481014No Hit
ACCGATTAGAGGCGTTTTATGATAATCC20.10126582278481014No Hit
TGCGTACCCGACGACCAAAATTAGGGTC20.10126582278481014No Hit
TCTGCCGCTGATAAAGGAAAGGATACTC20.10126582278481014No Hit
CGTTTATCGCAATCTGCCGACCACTCGC20.10126582278481014No Hit
GATTTGAGAATCAAAAAGAGCTTACTAA20.10126582278481014No Hit
ATTTTAAAGCGCCGTGGATGCCTGACCG20.10126582278481014No Hit
AACCTCACACTCAATCTTTTATCACGAA20.10126582278481014No Hit
CGGCTCATTCTGATTCTGAACAGCTTCT20.10126582278481014No Hit
CAATTCATCCATTAACTTCTCAGTAACA20.10126582278481014No Hit
CGGATTTGAGAATCAAAAAGAGCTTACT20.10126582278481014No Hit
GGCCTGTCTCATCATGGAAGGCGCTGAA20.10126582278481014No Hit
GCTCTTTTTGATTCTCAAATCCGGCGTC20.10126582278481014No Hit
GCTGAACGCCCTCTTAAGGATATTCGCG20.10126582278481014No Hit
CATGGTCAATATAACCAGTAGTGTTAAC20.10126582278481014No Hit
CAATACCGCCAGCAATAGCACCAAACAT20.10126582278481014No Hit
GTACGGGGAAGGACGTCAATAGTCACAC20.10126582278481014No Hit
CATCTGAATGCAATGAAGAAAACCACCA20.10126582278481014No Hit
TACAGTCGTTTGTGGATGAATACTGCCA20.10126582278481014No Hit
AGTTTGAATATTAGACATAATTTATCCT20.10126582278481014No Hit
GGTTCTCACTTCTGTTACTCCAGCTTCT20.10126582278481014No Hit
CATAGAAATTTCACGCGGCGGCAAGTTG20.10126582278481014No Hit
GAGCGTCCGGTTAAAGCCGCTGAATTGT20.10126582278481014No Hit
TATTCAAACTGGCGCCGAGCGTATGCCG20.10126582278481014No Hit
CCAGCAAGGAAGCCAAGATGGGAAAGGT20.10126582278481014No Hit
TGCCATGCTCAGGAACAAAGAAACGCGG20.10126582278481014No Hit
ATTGCTGCCGTCATTGCTTATTATGTTC20.10126582278481014No Hit
CGAAGATGATTTCGATTTTCTGACGAGT20.10126582278481014No Hit
GCTCGCGTTGCGTCTATTATGGAAAACA20.10126582278481014No Hit
TTTCATGGTATTGATAAAGCTGTTGCCG20.10126582278481014No Hit
CAAGTTGGGGGAGCACATTGTAGCATTG20.10126582278481014No Hit
TTTTTTGCGTTGTGGATGAATACTGCCA20.10126582278481014No Hit
ATGAGTTTGTATCTGTTACTGAGAAGTT20.10126582278481014No Hit
GATTTTATTGGTATCAGGGTTAATCGTG20.10126582278481014No Hit
CCGGTTATCGCTGGCGACTCCTTCGAGA20.10126582278481014No Hit
TTCGACTCATCAGAAATATCCGAAAGTG20.10126582278481014No Hit
CATTTGTTTCAGGGTTATTTGAATATCT20.10126582278481014No Hit
CTGGCGGCGATTGCGTACCCGACGACCA20.10126582278481014No Hit
GAAACATGATTAAACTCCTAAGCAGAAA20.10126582278481014No Hit
AGTGACGACATTAGAAATATCCTTTGCA20.10126582278481014No Hit
TGGCGGCGATTGCGTACCCGACGACCAA20.10126582278481014No Hit
ATAATAACCACCATCATGGCGACCATCC20.10126582278481014No Hit
TCGCTACTTCCCAAGAAGCTGTTCAGAA20.10126582278481014No Hit
CACATAGAAACCAACAGCCATATAACTG20.10126582278481014No Hit
AATAGTCACACAGTCCTTGACGGTATAA20.10126582278481014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCCA501.096123E-822.022
ATGAATA501.096123E-822.016
TACTGCC501.096123E-822.021
TGTGGAT501.096123E-822.011
AATACTG501.096123E-822.019
GAATACT501.096123E-822.018
ATACTGC501.096123E-822.020
TTGTGGA250.001775162922.010
GTGGATG501.096123E-822.012
TGAATAC501.096123E-822.017
GGATGAA553.034438E-820.014
GATGAAT553.034438E-820.015
TGGATGA553.034438E-820.013
Produced by FastQC (version 0.11.5)