FastQCFastQC Report
Fri 28 Aug 2020
000000000-G69NY_l01_n01_WGS17-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NY_l01_n01_WGS17-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125252
Sequences flagged as poor quality0
Sequence length28
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTCCGCTTGTGGATGAATACTGCCA1610.1285408616229681No Hit
TAGTTTTTTATGTGGATGAATACTGCCA1600.12774247117810494No Hit
GTATCTCTTTTGTGGATGAATACTGCCA1580.12614569028837863No Hit
GTATCCTCGGTGTGGATGAATACTGCCA1400.11177466228084183No Hit
TCCGCATTAATGTGGATGAATACTGCCA1290.1029923673873471No Hit
GTAGCAGTTATGTGGATGAATACTGCCA1270.1013955864976208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTGT555.140464E-922.0000027
GATGTGG5300.022.0000029
GACTGTG555.140464E-922.0000028
GGGGTTG750.022.0000026
TCTCTGT750.022.0000027
TACTTGT555.140464E-922.0000027
ATCTGTG1500.022.0000028
TTATGGG250.002567664522.04
ACAGTGT403.5728917E-622.07
TGGTTTG250.002567664522.06
TTTTGCT250.002567664522.05
TGGTTGT353.1857864E-522.07
TCCTTGT302.85292E-422.07
TCCCATG250.002567664522.06
GTGTTTG504.541107E-822.06
ATCTCTA250.002567664522.04
TAAACTG250.002567664522.06
CGATATA302.85292E-422.04
TAAATTG250.002567664522.06
GGGTTGT1050.022.07
Produced by FastQC (version 0.11.5)